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Invitrogen
Glutaminase Polyclonal Antibody
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FIGURE: 1 / 4
Glutaminase Antibody (PA5-115296) in ICC/IF
Immunocytochemistry analysis of Glutaminase in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115296) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-115296
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GLS(Accession O94925), corresponding to amino acid residues V592-Y642.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Glutaminase.
Target Information
Glutaminase is a phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. It is primarily expressed in the brain and kidney and plays an essential role in generating energy for metabolism, synthesizing neurotransmitter glutamate, and maintaing acid-based balance in the kidney. There are alternate splicing results in multiple transcript variants in the Glutaminase gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686O15119; FLJ10358; glutaminase C; glutaminase, phosphate-activated; K-glutaminase; kidney-type glutaminase; L-glutamine amidohydrolase; pot. glutaminase (EC 3.5.1.2); putative; unnamed protein product
Gene Aliases: 6330442B14; AAD20; B230365M23Rik; CASGID; DEE71; EIEE71; GAC; GAM; GDPAG; GLS1; Glut; KGA; RATGLUT
UniProt ID: (Human) O94925, (Rat) P13264, (Mouse) D3Z7P3
Entrez Gene ID: (Human) 2744, (Rat) 24398, (Mouse) 14660
cellular amino acid metabolic process
glutamine metabolic process
glutamine catabolic process
protein homotetramerization
negative regulation of L-glutamine biosynthetic process
glutamate homeostasis
L-glutamate biosynthetic process
suckling behavior
regulation of respiratory gaseous exchange by neurological system process
chemical synaptic transmission
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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