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Invitrogen
LMX1A Polyclonal Antibody
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FIGURE: 1 / 5
LMX1A Antibody (PA5-115517) in ICC/IF
Immunocytochemistry analysis of LMX1A in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115517) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-115517
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human LMX1A(Accession Q8TE12), corresponding to amino acid residues R152-L202.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total LMX1A.
Target Information
The LIM homeobox transcription factor 1 (LMX1A) belongs to the LIM-homeodomain family. Members of this family are known to be important for pattern formation during development. LMX1A binds an A/T-rich sequence and functions in the nucleus as a transcriptional activator to the insulin gene promoter. In the developing embryo, LMX1A is expressed along the neuraxis and leads to the development of the roof plate of the vertebrae. While LMX1A is crucial for the differentiation of dopamine neurons, the related LMX1B appears to be important for both the development and the survival of these cells. It has been suggested that LMX1A might be a useful biomarker or risk factor in tumor aggressiveness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: LIM/homeobox protein 1.1; LIM/homeobox protein LMX1A; LMX-1; LMX-1.1; MGC87616; shaker short-tail; unnamed protein product
Gene Aliases: DFNA7; dr; dreher; LMX1; LMX1.1; sst
UniProt ID: (Human) Q8TE12, (Mouse) Q9JKU8
Entrez Gene ID: (Human) 4009, (Rat) 289201, (Mouse) 110648
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
protein binding
sequence-specific double-stranded DNA binding
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
synapse organization
dopaminergic neuron differentiation
midbrain dopaminergic neuron differentiation
regulation of cell growth
axon guidance
central nervous system development
memory
locomotory behavior
regulation of gene expression
dentate gyrus development
cerebellum development
hippocampus development
central nervous system neuron differentiation
midbrain development
olfactory behavior
negative regulation of neuron differentiation
positive regulation of canonical Wnt signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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