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Invitrogen
Nkx2.2 Polyclonal Antibody
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FIGURE: 1 / 2
Nkx2.2 Antibody (PA5-115656) in ICC/IF
Immunocytochemistry analysis of Nkx2.2 in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115656) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-115656
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NKX2-2(Accession O95096), corresponding to amino acid residues H88-A138.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total NKX22.
Target Information
The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Drosophila NK2 transcription factor related, locus 2; glycoprotein GP330, renal; homeobox protein NK-2 homolog B; homeobox protein Nkx2-2; NK2 transcription factor related, locus 2; NK2 transcription factor-like protein B; Nkx2.2; unnamed protein product
Gene Aliases: Nkx-2.2; NKX2.2; NKX2B; tinman
UniProt ID: (Human) O95096, (Mouse) P42586
Entrez Gene ID: (Human) 4821, (Rat) 366214, (Mouse) 18088
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
type B pancreatic cell development
pancreatic A cell fate commitment
type B pancreatic cell fate commitment
pancreatic PP cell fate commitment
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
smoothened signaling pathway
nervous system development
response to glucose
positive regulation of gene expression
oligodendrocyte development
spinal cord motor neuron differentiation
spinal cord oligodendrocyte cell differentiation
spinal cord oligodendrocyte cell fate specification
optic nerve development
cell differentiation
endocrine pancreas development
response to progesterone
positive regulation of cell differentiation
negative regulation of neuron differentiation
positive regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
cell development
digestive tract development
neuron fate specification
astrocyte differentiation
positive regulation of oligodendrocyte differentiation
ventral spinal cord interneuron fate determination
brain development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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