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Invitrogen
P2X2 Polyclonal Antibody
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FIGURE: 1 / 4
P2X2 Antibody (PA5-115706) in ICC/IF
Immunocytochemistry analysis of P2X2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115706) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-115706
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human P2RX2(Accession Q9UBL9), corresponding to amino acid residues H408-Q458.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total P2X2.
Target Information
P2rx2 are members of the ligand-gated ion channel family that open in response to extracellular ATP. Each receptor is made up of a trimer of subunits (P2X1-7) all of which share the common structure of two transmembrane domains. P2rx2 functions as a ligand-gated ion channel and binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Six transcript variants encoding six distinct isoforms have been identified for P2rx2. P2rx2 is involved in a variety of cellular responses, such as excitatory postsynaptic responses in sensory neurons, neuromuscular junctions (NMJ) formation, hearing, perception of taste and peristalsis. In the inner ear, P2rx2 regulates sound transduction and auditory neurotransmission, outer hair cell electromotility, inner ear gap junctions, and K(+) recycling. P2rx2 also mediates synaptic transmission between neurons and from neurons to smooth muscle. Diseases associated with P2RX2 include Deafness, Autosomal Dominant 41 and Dfna41 Nonsyndromic Hearing Loss And Deafness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP receptor; ligand-gated ion channel; ligand-gated ion channel subunit; ligand-gated ion channel; alternatively spliced; MGC129601; P2X ligand-gated ion channel; P2X Receptor, subunit 2; purinergic receptor P2X, ligand gated ion channel, 2; unnamed protein product
Gene Aliases: DFNA41; P2X2; P2X2a
UniProt ID: (Human) Q9UBL9, (Mouse) Q8K3P1
Entrez Gene ID: (Human) 22953, (Mouse) 231602
purinergic nucleotide receptor activity
extracellular ATP-gated cation channel activity
ion channel activity
ATP binding
ligand-gated ion channel activity
identical protein binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
copper ion binding
zinc ion binding
nickel cation binding
phosphatidylinositol binding
macromolecular complex binding
mercury ion binding
cadmium ion binding
cobalt ion binding
response to ischemia
ion transport
sensory perception of sound
positive regulation of calcium ion transport into cytosol
response to ATP
purinergic nucleotide receptor signaling pathway
positive regulation of calcium-mediated signaling
excitatory postsynaptic potential
calcium ion transmembrane transport
cation transmembrane transport
response to hypoxia
detection of hypoxic conditions in blood by carotid body chemoreceptor signaling
chemical synaptic transmission
neuromuscular synaptic transmission
neuromuscular junction development
response to carbohydrate
urinary bladder smooth muscle contraction
neuronal action potential
peristalsis
positive regulation of transcription from RNA polymerase II promoter
behavioral response to pain
skeletal muscle fiber development
sensory perception of taste
regulation of presynaptic cytosolic calcium ion concentration
regulation of synaptic vesicle exocytosis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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