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Antibody Testing Data (3)

Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

Actin Antibody (PA5-11571) in IHC (P)

Immunohistochemistry analysis of Actin in formalin-fixed and paraffin-embedded human skeletal muscle tissue. Samples were incubated with Actin polyclonal antibody (Product # PA5-11571) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image Actin Polyclonal Antibody

Product Details

PA5-11571

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Product Specifications

Species Reactivity

Human

Published species

Plant

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 189-218 amino acids from the central region of human Actin (ACTB/ACTC)

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

800 µg

Purification

Ammonium sulfate precipitation, Size-exclusion - Dialysis

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Ambient (domestic); Wet ice (international)

AB_2223355

Product Specific Information

This antibody is predicted to react with bovine, chicken, canine, equine, hamster, mouse, porcine, non-human primate, rat, rabbit and Xenopus, yeast and zebrafish based on sequence homology.

Target Information

Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ACTA1; ACTA2; ACTC; ACTG2; Actin is a protein with a high degree of evolutionary conservation that undergoes polymerization to generate filaments, which subsequently assemble into interconnected networks within the cytoplasm of cells.; actin, alpha 2, smooth muscle, aorta; actin, gamma 2, smooth muscle, enteric; actin-like protein; alfa sma; alpha smooth muscle actin; alpha-actin; alpha-actin-3; alpha-cardiac actin; alpha-sma; aSMA; aSmooth Muscle Actin; Atcb; beta cytoskeletal actin; beta-actin; cell growth-inhibiting gene 46 protein; cytoskeletal gamma-actin; epididymis luminal protein 176; gamma-actin; GIG46; I(2)-actin; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; unnamed protein product

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Gene Aliases: ACT; ACTA; ACTA3; ACTC; ACTE; ACTG; ACTL3; ACTSA; ACTSG; ASD5; ASMA; BKRNS; BNS; BRWS1; CFTD; CFTD1; CFTDM; CMD1R; CMH11; CMYO2A; CMYO2B; CMYO2C; CMYP2A; CMYP2B; CMYP2C; CSMH; DDS1; DFNA20; DFNA26; HEL-176; LVNC4; MMIHS5; MPFD; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMDYS; THC8; VSCM; VSCM1

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UniProt ID: (Human) P68133, (Human) P62736, (Human) P60709, (Human) P68032, (Human) P63261, (Human) P63267

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Entrez Gene ID: (Human) 58, (Human) 59, (Human) 60, (Human) 70, (Human) 71, (Human) 72

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Function(s)

structural constituent of cytoskeleton protein binding ATP binding ATPase activity myosin binding ADP binding protein kinase binding kinesin binding nitric-oxide synthase regulator activity Tat protein binding nucleosomal DNA binding identical protein binding tau protein binding nitric-oxide synthase binding structural constituent of postsynaptic actin cytoskeleton transporter regulator activity microfilament motor activity profilin binding ubiquitin protein ligase binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Actin Polyclonal Antibody

Actin Antibody (PA5-11571) in IHC (P)

Product Details

PA5-11571

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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