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Invitrogen
THRAP5 Polyclonal Antibody
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FIGURE: 1 / 2
THRAP5 Antibody (PA5-116039) in IHC (P)
Immunohistochemistry analysis of THRAP5 in mouse lung tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-116039) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-116039
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MED16(Accession Q9Y2X0), corresponding to amino acid residues Q619-K669.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total THRAP5.
Target Information
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MED16_i10; alternatively spliced; MED16_i11; alternatively spliced; MED16_i12; alternatively spliced; MED16_i13; alternatively spliced; MED16_i14; alternatively spliced; MED16_i15; alternatively spliced; MED16_i16; alternatively spliced; MED16_i17; alternatively spliced; MED16_i18; alternatively spliced; MED16_i19; alternatively spliced; MED16_i20; alternatively spliced; MED16_i21; alternatively spliced; MED16_i22; alternatively spliced; MED16_i23; alternatively spliced; MED16_i24; alternatively spliced; MED16_i25; alternatively spliced; MED16_i26; alternatively spliced; MED16_i27; alternatively spliced; MED16_i28; alternatively spliced; MED16_i29; alternatively spliced; MED16_i2; alternatively spliced; MED16_i30; alternatively spliced; MED16_i31; alternatively spliced; MED16_i32; alternatively spliced; MED16_i33; alternatively spliced; MED16_i34; alternatively spliced; MED16_i35; alternatively spliced; MED16_i3; alternatively spliced; MED16_i4; alternatively spliced; MED16_i5; alternatively spliced; MED16_i6; alternatively spliced; MED16_i7; alternatively spliced; MED16_i8; alternatively spliced; MED16_i9; alternatively spliced; thyroid hormone receptor associated protein 5; thyroid hormone receptor-associated protein 5; thyroid hormone receptor-associated protein complex 95 kDa component; thyroid hormone receptor-associated protein, 95-kD subunit; unnamed protein product; vitamin D3 receptor-interacting protein complex 92 kDa component
Gene Aliases: 95kDa; A630083L04; DRIP92; GGNS; THRAP5; TRAP95
UniProt ID: (Human) Q9Y2X0, (Mouse) Q6PGF3
Entrez Gene ID: (Human) 10025, (Rat) 299607, (Mouse) 216154
transcription from RNA polymerase II promoter
biological_process
positive regulation of transcription, DNA-templated
positive regulation of transcription initiation from RNA polymerase II promoter
positive regulation of transcription elongation from RNA polymerase II promoter
RNA polymerase II transcriptional preinitiation complex assembly
regulation of transcription from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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