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          • Primary Antibodies ›
          • CRK Antibodies

          Invitrogen

          Crk Polyclonal Antibody

          View all (26) CRK antibodies

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          Cite Crk Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          Crk Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Crk Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Crk Antibody (PA5-116651) in WB

          Western blot analysis of Crk in extracts of various cell lines. Samples were incubated with Crk Polyclonal antibody (Product # PA5-116651) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Crk Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Crk Polyclonal Antibody

          Product Details

          PA5-116651

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein (or fragment).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.18 mg/mL

          Amount

          18 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2901282

          Product Specific Information

          Positive Samples: A-549, MCF7, BT-474, Mouse brain

          Immunogen sequence: MAGNFDSEER SSWYWGRLSR QEAVALLQGQ RHGVFLVRDS STSPGDYVLS VSENSRVSHY IINSSGPRPP VPPSPAQPPP GVSPSRLRIG DQEFDSLPAL LEFYKIHYLD TTTLIEPVSR SRQGSGVILR QEEAEYVRAL FDFNGNDEED LPFKKGDILR IRDKPEEQWW NAEDSEGKRG MIPVPYVEKY RPASASVSAL IGGNQEGSHP QPLGGPEPGP YAQPSVNTPL PNLQNGPIYA RVIQKRVPNA YDKTALALEV GELVKVTKIN VSGQWEGECN GKRGHFPFTH VRLLDQQNPD EDFS

          Target Information

          The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk- II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: avian sarcoma virus CT10 (v-crk) oncogene homolog; Crk3; CSAID-binding protein; CT-10 related kinase 3; cytokine suppressive anti-inflammatory drug binding protein; encoded by transcript variant 4; p38 MAP kinase; encoded by transcript variant 5; p38 MAP kinase; MAP kinase 14; MAP kinase Mxi2; MAP kinase p38 alpha; MAPK 14; MAPK14; MAX-interacting protein 2; mitogen-activated protein kinase p38 alpha; negative regulator of Crk tyrosine-phosphorylation; p38 MAP kinase; p38 MAP kinase alpha; p38 MAPK; p38 mitogen activated protein kinase; p38alpha Exip; proto-oncogene c-Crk; SAPK2a; CSAIDS binding protein 1; stress-activated protein kinase 2A; unnamed protein product; v-crk avian sarcoma virus CT10 oncogene homolog; v-crk sarcoma virus CT10 oncogene homolog (avian) isoform CRK_1; v-crk sarcoma virus CT10 oncogene homolog (avian) isoform CRK_2; v-crk sarcoma virus CT10 oncogene-like protein

          View more View less

          Gene Aliases: c-Crk; Crk-I; Crk-II; Crk-III; Crk1; Crk3; CRKII; CrkIII; Crko; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; p38; p38-alpha; p38a; p38ALPHA; p38MAPK; PRKM14; PRKM15; RK; SAPK2A

          View more View less

          UniProt ID: (Human) P46108, (Mouse) Q64010, (Human) Q16539, (Mouse) P47811

          View more View less

          Entrez Gene ID: (Human) 1398, (Mouse) 12928, (Human) 1432, (Mouse) 26416

          View more View less

          Function(s)
          phosphotyrosine binding insulin-like growth factor receptor binding protein binding cytoskeletal protein binding SH3 domain binding enzyme binding kinase binding protein domain specific binding receptor signaling complex scaffold activity protein binding, bridging receptor tyrosine kinase binding ubiquitin protein ligase binding signaling adaptor activity SH2 domain binding protein phosphorylated amino acid binding scaffold protein binding protein tyrosine kinase binding ephrin receptor binding protein kinase activity protein serine/threonine kinase activity MAP kinase activity MAP kinase kinase activity ATP binding protein phosphatase binding mitogen-activated protein kinase p38 binding NFAT protein binding protein serine kinase activity kinase activity
          Process(es)
          MAPK cascade negative regulation of inflammatory response to antigenic stimulus regulation of transcription from RNA polymerase II promoter chemotaxis signal transduction cell surface receptor signaling pathway positive regulation of gene expression positive regulation of myotube differentiation platelet activation osteoclast differentiation stress-activated protein kinase signaling cascade positive regulation of cyclase activity positive regulation of interleukin-12 production negative regulation of hippo signaling intracellular signal transduction cellular response to vascular endothelial growth factor stimulus p38MAPK cascade signal transduction in response to DNA damage positive regulation of blood vessel endothelial cell migration positive regulation of erythrocyte differentiation positive regulation of myoblast differentiation vascular endothelial growth factor receptor signaling pathway positive regulation of muscle cell differentiation stress-activated MAPK cascade 3'-UTR-mediated mRNA stabilization cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to ionizing radiation cellular response to UV-B cellular senescence stress-induced premature senescence cellular response to virus regulation of cytokine production involved in inflammatory response positive regulation of myoblast fusion positive regulation of reactive oxygen species metabolic process DNA damage checkpoint cell morphogenesis cartilage condensation angiogenesis osteoblast differentiation placenta development response to dietary excess chondrocyte differentiation glucose metabolic process regulation of transcription, DNA-templated protein phosphorylation cellular response to DNA damage stimulus transmembrane receptor protein serine/threonine kinase signaling pathway skeletal muscle tissue development myoblast differentiation involved in skeletal muscle regeneration peptidyl-serine phosphorylation fatty acid oxidation regulation of ossification lipopolysaccharide-mediated signaling pathway response to muramyl dipeptide response to lipopolysaccharide response to insulin tumor necrosis factor-mediated signaling pathway response to muscle stretch positive regulation of protein import into nucleus positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import protein autophosphorylation striated muscle cell differentiation protein maturation regulation of cardiac muscle cell proliferation positive regulation of cardiac muscle cell proliferation bone development pyroptosis cellular response to tumor necrosis factor negative regulation of canonical Wnt signaling pathway positive regulation of brown fat cell differentiation regulation of synaptic membrane adhesion NLRP1 inflammasome complex assembly neuron migration response to yeast regulation of leukocyte migration lipid metabolic process enzyme linked receptor protein signaling pathway synapse assembly cell proliferation regulation of cell shape regulation of signal transduction positive regulation of smooth muscle cell migration dendrite development cell migration hippocampus development cerebral cortex development establishment of cell polarity actin cytoskeleton organization positive regulation of cell growth regulation of actin cytoskeleton organization regulation of cell adhesion mediated by integrin regulation of Rac protein signal transduction positive regulation of Rac protein signal transduction helper T cell diapedesis response to hepatocyte growth factor reelin-mediated signaling pathway response to hydrogen peroxide negative regulation of natural killer cell mediated cytotoxicity positive regulation of JNK cascade ephrin receptor signaling pathway regulation of dendrite development cell chemotaxis negative regulation of wound healing response to cholecystokinin cellular response to transforming growth factor beta stimulus cellular response to nitric oxide protein localization to membrane activation of GTPase activity postsynaptic specialization assembly cerebellar neuron development positive regulation of substrate adhesion-dependent cell spreading response to peptide regulation of intracellular signal transduction cellular response to nerve growth factor stimulus cellular response to insulin-like growth factor stimulus cellular response to endothelin negative regulation of cell motility regulation of T cell migration cell adhesion regulation of wound healing regulation of cell motility
          It has to be done as per old AB suggested Products section.

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