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Invitrogen
ADARB1 Polyclonal Antibody
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FIGURE: 1 / 1
ADARB1 Antibody (PA5-116693) in WB
Western blot analysis of ADARB1 in extracts of various cell lines. Samples were incubated with ADARB1 Polyclonal antibody (Product # PA5-116693) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-116693
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.94 mg/mL
Amount
94 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: U-251MG, HeLa, Jurkat, HepG2, mouse brain, mouse lung, mouse testis, rat brain
Immunogen sequence: FETPDKAEPP FYVGSNGDDS FSSSGDLSLS ASPVPASLAQ PPLPVLPPFP PPSGKNPVMI LNELRPGLKY DFLSESGESH AKSFVMSVVV DGQFFEGSGR NKKLAKARAA QSALAAIFNL HLDQTPSRQP IPSEGLQLHL PQVLADAVSR LVLGKFGDLT DNFSSPHARR KVLAGVVMTT GTDVKDAKVI SVSTGTKCIN G
Target Information
Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADAR2; RNA editase; ADAR2a; adenosine deaminase acting on RNA; adenosine deaminase, RNA-specific, B1 (homolog of rat RED1); double stranded RNA specific deaminase RED1; dsRNA adenosine deaminase; dsRNA adenosine deaminase DRADA2; homologous to rat RED1; RNA editase; RNA editase A-I; RNA editing deaminase 1; RNA editing deaminase of glutamate receptors; RNA-editing deaminase 1; RNA-editing enzyme 1; similar to rat RED1; unnamed protein product
Gene Aliases: 1700057H01Rik; ADAR2; D10Bwg0447e; DRABA2; DRADA2; NEDHYMS; RED1
UniProt ID: (Human) P78563, (Mouse) Q91ZS8, (Rat) P51400
Entrez Gene ID: (Human) 104, (Mouse) 110532, (Rat) 25367
adenosine to inosine editing
RNA processing
mRNA processing
neuromuscular synaptic transmission
negative regulation of cell proliferation
mRNA metabolic process
base conversion or substitution editing
mRNA modification
facial nerve morphogenesis
hypoglossal nerve morphogenesis
spinal cord ventral commissure morphogenesis
negative regulation of cell migration
multicellular organism growth
negative regulation of protein kinase activity by regulation of protein phosphorylation
positive regulation of viral genome replication
positive regulation of mRNA processing
neuromuscular process controlling posture
regulation of cell cycle
innervation
muscle tissue morphogenesis
motor behavior
motor neuron apoptotic process
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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