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Invitrogen
CD47 Polyclonal Antibody
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FIGURE: 1 / 2
CD47 Antibody (PA5-116827) in WB
Western blot analysis of CD47 in extracts of various cell lines. Samples were incubated with CD47 Polyclonal antibody (Product # PA5-116827) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-116827
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.78 mg/mL
Amount
178 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: MCF7, Jurkat, U-251MG, Mouse spleen, Mouse kidney, Rat kidney
Target Information
CD47, known as integrin-associated protein (IAP), is a glycosylated transmembrane glycoprotein with five domains, expressed widely across hematopoietic cells like T and B cells, monocytes, platelets, and erythrocytes, as well as non-hematopoietic cells. It interacts with integrins such as CD51/CD61 and CD41/CD61, and serves as a receptor for thrombospondin, mediating bi-directional signaling that affects neural synaptic activity and macrophage phagocytosis. CD47 acts as a ligand for CD172a (SIRP alpha), an inhibitory receptor on macrophages, preventing phagocytosis of CD47-positive cells. This interaction influences cell migration, B cell adhesion, T cell activation, and neuronal development, particularly in synapse-rich brain and retina regions. It also modulates chondrocyte responses to mechanical signals. T cell expression of CD47 can lead to activation or apoptosis in the presence of thrombospondin. Monoclonal antibody stimulation of CD47 has been shown to induce CD4+CD25- suppressive activity and increase Foxp3 expression. CD47's role in membrane transport, signal transduction, and its broad tissue distribution highlight its significance in various physiological processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; Rh-related antigen; sCD47; soluble CD 47; soluble CD47; unnamed protein product
Gene Aliases: 9130415E20Rik; B430305P08Rik; IAP; Itgp; MER6; OA3
UniProt ID: (Human) Q08722, (Mouse) Q61735
Entrez Gene ID: (Human) 961, (Mouse) 16423
positive regulation of immune system process
inflammatory response
opsonization
positive regulation of cell proliferation
response to bacterium
cell migration
positive regulation of cell-cell adhesion
regulation of interferon-gamma production
regulation of interleukin-10 production
regulation of interleukin-12 production
regulation of interleukin-6 production
regulation of tumor necrosis factor production
heterotypic cell-cell adhesion
regulation of nitric oxide biosynthetic process
positive regulation of inflammatory response
negative regulation of phagocytosis
positive regulation of phagocytosis
positive regulation of T cell activation
positive regulation of stress fiber assembly
regulation of Fc receptor mediated stimulatory signaling pathway
positive regulation of activation-induced cell death of T cells
monocyte aggregation
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to interleukin-12
cell-cell adhesion
ATP export
regulation of synapse pruning
positive regulation of monocyte extravasation
integrin-mediated signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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