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Invitrogen
ULK1/ULK2 Polyclonal Antibody
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FIGURE: 1 / 1
ULK1/ULK2 Antibody (PA5-116888) in WB
Western blot analysis of ULK1/ULK2 in extracts of various cell lines. Samples were incubated with ULK1/ULK2 Polyclonal antibody (Product # PA5-116888) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-116888
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human ULK1/ULK2 (NP_0035561)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: RD, SH-SY5Y, HepG2
Immunogen sequence: MEPGRGGTET VGKFEFSRKD LIGHGAFAVV FKGRHREKHD LEVAVKCINK KNLAKSQTLL GKEIKILKEL KHENIVALYD FQEMANSVYL VMEYCNGGDL ADYLHAMRTL SEDTIRLFLQ QIAGAMRLLH SKGIIHRDLK PQNILLSNPA GRRANPNSIR VKIADFGFAR YLQSNMMAAT LCGSPMYMAP EVIMSQHYDG KADLWSIGTI VYQCLTGKAP FQASSPQDLR LFYEKNKTLV PTIPRETSAP LRQLLLALLQ RNHKDRMDFD EFFHHPFLDA SPS
Target Information
Mammalian ULK1 (unc-51 like kinase 1) and ULK2, Caenorhabditis elegans UNC-51, and Drosophila melanogaster Atg1 are serine/threonine kinases that regulate flux through the autophagy pathway in response to various types of cellular stress.ULK1 and ULK2 all have different tissue-specific expressions partially contributing to diverse and ULK-specific interaction networks in various tissues.ULK1 (or its close family member ULK2) interacts with several other proteins to form a large complex that is required for the formation of an autophagosome. The other proteins that complex with ULK1/ULK2 include ATG13, ATG101, and RB1CC1 (FIP200/ATG17).AMPK, activated during low nutrient conditions, directly phophorylates ULK1 at multiple sites including Ser317, Ser555, and Ser777. Conversely, mTOR, which is a regulator of cell growth and is an inhibitor of autophagy, phosphorylates ULK1 at Ser757 and disrupts the interaction between ULK1 and AMPK.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATG1 autophagy related 1 homolog; autophagy-related protein 1 homolog; unnamed protein product
Gene Aliases: ATG1; ATG1A; ATG1B; hATG1; UNC51; Unc51.1; Unc51.2
UniProt ID: (Human) O75385, (Human) Q8IYT8
Entrez Gene ID: (Human) 8408, (Human) 9706
autophagosome assembly
mitophagy
protein phosphorylation
autophagy
signal transduction
axonogenesis
protein localization
negative regulation of cell proliferation
regulation of autophagy
positive regulation of autophagy
regulation of tumor necrosis factor-mediated signaling pathway
macroautophagy
regulation of macroautophagy
peptidyl-serine phosphorylation
neuron projection development
negative regulation of protein complex assembly
cellular response to nutrient levels
cellular response to stress
piecemeal microautophagy of nucleus
response to starvation
protein autophosphorylation
negative regulation of collateral sprouting
axon extension
reticulophagy
regulation of protein lipidation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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