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          • Primary Antibodies ›
          • c-Myc Antibodies

          Invitrogen

          Phospho-c-Myc (Ser62) Polyclonal Antibody

          View all (149) c-Myc antibodies

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          Cite Phospho-c-Myc (Ser62) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (4)
          Phospho-c-Myc (Ser62) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Phospho-c-Myc (Ser62) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          Phospho-c-Myc (Ser62) Antibody (PA5-117178) in IHC (P)

          Immunohistochemistry analysis of Phospho-c-Myc (Ser62) in paraffin-embedded human appendix. Samples were incubated with Phospho-c-Myc (Ser62) Polyclonal antibody (Product # PA5-117178) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-c-Myc (Ser62) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-c-Myc (Ser62) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-c-Myc (Ser62) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-c-Myc (Ser62) Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Phospho-c-Myc (Ser62) Polyclonal Antibody

          Product Details

          PA5-117178

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A phospho specific peptide corresponding to residues surrounding S62 of human MYC (NP_0013417991)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.32 mg/mL

          Amount

          32 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.09% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2901808

          Product Specific Information

          Positive Samples: NIH/3T3

          Immunogen sequence: PLSPSR

          Target Information

          The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Avian myelocytomatosis viral (v-myc) oncogene homolog; avian myelocytomatosis viral oncogene homolog; c Myc; c-myc; c-myc epitope tag; c-myc protein, first expressed; c-myc proto-oncogene; class E basic helix-loop-helix protein 39; cmyc; DNA binding protein and transcription regulator; Myc protein; myc-related translation/localization regulatory factor; myelocytomatosis oncogene; myelocytomatosis viral oncogene homolog; ORF 114; ORF 114; putative; ORF 118; proto-oncogene c-Myc; transcription factor p64; unnamed protein product; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog

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          Gene Aliases: bHLHe39; c-Myc; mMyc; MRTL; Myc2; MYCC; Niard; Nird; RNCMYC

          View more View less

          UniProt ID: (Human) P01106, (Mouse) P01108, (Rat) P09416

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          Entrez Gene ID: (Human) 4609, (Mouse) 17869, (Rat) 24577

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          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter sequence-specific DNA binding transcription cofactor binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding identical protein binding macromolecular complex binding protein dimerization activity E-box binding DNA-binding transcription factor binding SCF ubiquitin ligase complex binding core promoter proximal region sequence-specific DNA binding double-stranded DNA binding ubiquitin protein ligase binding sequence-specific DNA binding mRNA binding eukaryotic initiation factor eIF2 binding transcription regulator activator activity
          Process(es)
          G1/S transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter MAPK cascade branching involved in ureteric bud morphogenesis positive regulation of mesenchymal cell proliferation chromatin remodeling regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cellular iron ion homeostasis cellular response to DNA damage stimulus positive regulation of cell proliferation response to xenobiotic stimulus regulation of gene expression regulation of cell cycle process positive regulation of gene expression rRNA metabolic process regulation of telomere maintenance positive regulation of telomere maintenance negative regulation of stress-activated MAPK cascade protein-DNA complex disassembly cellular response to UV negative regulation of apoptotic process hypermethylation of CpG island fibroblast apoptotic process negative regulation of monocyte differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation negative regulation of fibroblast proliferation positive regulation of epithelial cell proliferation chromosome organization negative regulation of cell division negative regulation of transcription initiation from RNA polymerase II promoter ERK1 and ERK2 cascade response to growth factor cellular response to hypoxia cellular response to xenobiotic stimulus positive regulation of metanephric cap mesenchymal cell proliferation positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of somatic stem cell population maintenance re-entry into mitotic cell cycle B cell apoptotic process NK T cell proliferation regulation of oxidative phosphorylation positive regulation of B cell apoptotic process glucose metabolic process transcription, DNA-templated DNA-templated transcription, initiation transcription from RNA polymerase II promoter pyruvate transport amino acid transport inner mitochondrial membrane organization regulation of mitotic cell cycle intrinsic apoptotic signaling pathway in response to DNA damage response to radiation negative regulation of gene expression positive regulation of mitochondrial membrane potential positive regulation of smooth muscle cell migration Wnt signaling pathway protein processing cellular response to interferon-alpha skeletal muscle cell differentiation middle ear morphogenesis regulation of apoptotic process positive regulation of catalytic activity proteasome-mediated ubiquitin-dependent protein catabolic process response to alkaloid pigmentation positive regulation of cell cycle positive regulation of glycolytic process negative regulation of glucose import lactic acid secretion positive regulation of smooth muscle cell proliferation skeletal system morphogenesis detection of mechanical stimulus involved in sensory perception of sound positive regulation of glial cell proliferation positive regulation of transcription initiation from RNA polymerase II promoter positive regulation of cellular respiration positive regulation of oxidative phosphorylation negative regulation of epithelial cell apoptotic process positive regulation of ATP biosynthetic process positive regulation of apoptotic signaling pathway ovarian follicle development in utero embryonic development hypothalamus development response to estradiol cellular response to insulin stimulus cellular response to platelet-derived growth factor stimulus cellular response to fibroblast growth factor stimulus response to human chorionic gonadotropin G0 to G1 transition response to ethanol myoblast proliferation cellular response to retinoic acid cellular response to carbohydrate stimulus cellular response to glucose stimulus cellular response to cytokine stimulus cellular response to interferon-gamma cellular response to interleukin-1 cellular response to epidermal growth factor stimulus cellular response to growth hormone stimulus cellular response to estrogen stimulus cellular response to testosterone stimulus cellular response to cycloheximide cellular response to hydrostatic pressure liver regeneration cellular response to arsenite(3-) cellular response to angiotensin cellular response to putrescine cellular response to dimethyl sulfoxide cellular response to phorbol 13-acetate 12-myristate cellular response to prolactin cellular response to lectin cellular response to endothelin
          It has to be done as per old AB suggested Products section.

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