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Invitrogen
RIG-I Polyclonal Antibody
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FIGURE: 1 / 5
RIG-I Antibody (PA5-11726) in ICC/IF
Immunofluorescence analysis of Probable ATP-dependent RNA helicase DDX58 was performed using 70% confluent log phase THP-1 cells and THP-1 cells treated with 1 µg/mL of LPS for 18 h. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RIG-I Polyclonal Antibody (Product # PA5-11726) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heav... View More
Product Details
PA5-11726
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 894-925 amino acids from the C-terminal region of human RIG-I
Conjugate
Form
Liquid
Purification
Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Retinoic acid-inducible gene I, RIG-I is a pattern recognition receptor (PRR) involved in the recognition of viral dsRNA. Along with MDA5, RIG-I detects viral dsRNA and activates the innate immune response. Both MDA5 and RIG-I are RNA helicases and they perform overlapping as well as distinct roles. RIG-I is activated by dsRNAs without a 5'-triphosphate end and short dsRNAs, whereas MDA5 is activated by long dsRNAs. Once activated, both proteins signal through IPS-1 activating transcription factors NF-kappaB and IRF-3 (1) and ultimately activating apoptosis, cytokine signaling, and inflammation. RIG-I is essential for signaling by influenza A, influenza B, human respiratory syncytial virus (3), paromyxoviruses, Japanese encephalitis virus, and West Nile virus. MicroRNA-146a has been implicated in feedback inhibition of RIG-I-dependant antiviral response by negatively regulating RIG-I targets TRAF6, IRAK1, and IRAK2. Recent evidence has implicated RIG-I in the detection of cytosolic DNA through RNA polymerase III activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antiviral innate immune response receptor RIG-I; ATP-dependent RNA helicase DDX58; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD box protein 58; DEAD-box protein 58; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; DEAD/H box polypeptide RIG-I; DExD/H-box helicase 58; probable ATP-dependent RNA helicase DDX58; Retinoic acid-inducible gene 1 protein; Retinoic acid-inducible gene I protein; retinoic acid-inducible gene-I; RIG-1; RIG-I; RIG-I-like receptor 1; RLR-1; RNA helicase RIG-I; RNA sensor RIG-I; unnamed protein product
Gene Aliases: 6430573D20Rik; C330021E21; DDX58; RIG-I; RIG1; RIGI; RLR-1; SGMRT2
UniProt ID: (Mouse) Q6Q899, (Human) O95786
Entrez Gene ID: (Mouse) 230073, (Human) 23586
nucleotide binding
nucleic acid binding
double-stranded DNA binding
RNA binding
double-stranded RNA binding
single-stranded RNA binding
helicase activity
protein binding
ATP binding
zinc ion binding
hydrolase activity
hydrolase activity, acting on acid anhydrides
identical protein binding
metal ion binding
RNA helicase activity
GTP binding
ATPase activity
ubiquitin protein ligase binding
pattern recognition receptor activity
positive regulation of defense response to virus by host
immune system process
detection of virus
response to virus
positive regulation of gene expression
regulation of cell migration
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
RIG-I signaling pathway
positive regulation of transcription factor import into nucleus
response to exogenous dsRNA
innate immune response
positive regulation of transcription from RNA polymerase II promoter
defense response to virus
positive regulation of immune system process
positive regulation of myeloid dendritic cell cytokine production
cytoplasmic pattern recognition receptor signaling pathway
mRNA transcription
gene expression
positive regulation of tumor necrosis factor production
regulation of type III interferon production
positive regulation of response to cytokine stimulus
cellular response to exogenous dsRNA
antiviral innate immune response
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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