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Invitrogen
WASH1 Polyclonal Antibody
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FIGURE: 1 / 2
WASH1 Antibody (PA5-118357) in WB
Western Blot using WASH1 Polyclonal Antibody (Product # PA5-118357) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: U- 251 MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 70 kDa.
Product Details
PA5-118357
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived Human WASH1/WASHC1 fragment
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7
Contains
0.03% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
WASHC1 (WASH1) is a protein coding gene with endocytosis as a related pathway. Acts as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Its assembly in the WASH core complex seems to inhibit its NPF activity and via WASHC2 is required for its membrane targeting. Involved in endocytic trafficking of EGF. Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration. In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation. Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex. Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: actin nucleation promoting factor; CXYorf1-like protein on chromosome 9; family with sequence similarity 39, member E; FLJ00075 protein; Protein FAM39E; unnamed protein product; WAS protein family homolog 1; WASH complex subunit 1
Gene Aliases: FAM39E; WASH; WASH1; WASHC1
UniProt ID: (Human) A8K0Z3
Entrez Gene ID: (Human) 100287171
exocytosis
negative regulation of autophagy
protein transport
endosomal transport
extracellular matrix disassembly
positive regulation of cell migration
positive regulation of pseudopodium assembly
regulation of protein ubiquitination
endocytic recycling
Arp2/3 complex-mediated actin nucleation
regulation of Arp2/3 complex-mediated actin nucleation
retrograde transport, endosome to Golgi
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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