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Invitrogen
Phospho-FOXO4 (Thr455) Polyclonal Antibody
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FIGURE: 1 / 7
Phospho-FOXO4 (Thr455) Antibody (PA5-118641) in IHC (P)
Immunohistochemical analysis of Phospho-FOXO4 (Thr455) in Human pancreas tissue using a Phospho-FOXO4 (Thr455) polyclonal antibody (Product # PA5-118641). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the se... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-118641
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human FOXO4(Accession P98177), corresponding to amino acid residues around phosphorylated Thr455.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of FOXO4 only when phosphorylated at Thr455.
Target Information
FOXO4 (Forkhead box O4) is encoded by the FOXO4 gene located on the X chromosome. It is also known as AFX1 or MLLT7 (Myeloid/Lymphoid or Mixed-Lineage Leukemia (Trithorax Homolog, Translocated To, 7) and is a member of the O class of forkhead/ winged helix family of transcription factors along with FOXO1, FOXO3 and FOXO6. FOXO4 is abundantly expressed in skeletal muscle and adipose tissue. FOXO4 binds to the Insulin Responsive Element (IRE) and activates downstream targets of Insulin/IGF1 signaling such as IGFBP1. However, the activation of another downstream target of the Insulin/IGF1 pathway, Akt, leads to phosphorylation of FOXO4 on specific Ser/Thr residues. This phosphorylation mediates binding of FOXO4 to 14-3-3 and eventual exit of FOXO4 from the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AFX; AFX1; AFXzeta; fork head domain transcription factor AFX1; forkhead protein; FOX04b; FOXO4b; member of FKHR-subclass forkhead/winged helix family; MGC120490; myeloid/lymphoid or mixed lineage-leukemia translocation to 7 homolog; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7; OTTHUMP00000023497; unnamed protein product
Gene Aliases: AFX; AFX1; Afxh; MLLT7; RGD1561201
UniProt ID: (Human) P98177, (Mouse) Q9WVH3
Entrez Gene ID: (Human) 4303, (Rat) 302415, (Mouse) 54601
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
nucleic acid binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
beta-catenin binding
enzyme binding
identical protein binding
sequence-specific DNA binding
DNA-binding transcription factor binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
response to oxidative stress
mitotic G2 DNA damage checkpoint
negative regulation of cell proliferation
insulin receptor signaling pathway
positive regulation of smooth muscle cell migration
negative regulation of angiogenesis
response to nutrient levels
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
stem cell differentiation
negative regulation of smooth muscle cell differentiation
negative regulation of G0 to G1 transition
response to water-immersion restraint stress
cellular response to oxidative stress
proteasome-mediated ubiquitin-dependent protein catabolic process
protein K48-linked ubiquitination
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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