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Phospho-53BP1 (Ser29) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-53BP1 (Ser29) Antibody (PA5-118674) in IHC (P)
Immunohistochemical analysis of Phospho-53BP1 (Ser29) in Human liver cancer tissue using a Phospho-53BP1 (Ser29) polyclonal antibody (Product # PA5-118674). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-118674
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TP53BP1(Accession Q12888), corresponding to amino acid residues around phosphorylated Ser29.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of 53BP1 only when phosphorylated at Ser29.
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 53BP1; FLJ41424; MGC138366; murine p53-binding protein; p53-binding protein 1; tumor protein 53-binding protein, 1; tumor suppressor p53-binding protein 1; unnamed protein product
Gene Aliases: 53BP1; m53BP1; p202; p53BP1; TDRD30; Tp53bp1
UniProt ID: (Human) Q12888, (Mouse) P70399
Entrez Gene ID: (Human) 7158, (Mouse) 27223
p53 binding
damaged DNA binding
transcription cofactor activity
transcription coactivator activity
protein binding
telomeric DNA binding
histone binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
ubiquitinated histone binding
histone H4K20me2 reader activity
histone reader activity
histone H4K20me methyltransferase activity
DNA damage checkpoint
double-strand break repair via nonhomologous end joining
chromatin organization
chromatin remodeling
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
positive regulation of isotype switching
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
protein homooligomerization
double-strand break repair via classical nonhomologous end joining
positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator
protein localization to site of double-strand break
negative regulation of double-strand break repair via homologous recombination
DNA repair
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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