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          • Primary Antibodies ›
          • IL-1 beta Antibodies

          Invitrogen

          IL-1 beta Polyclonal Antibody

          1 Published Figure
          2 References
          View all (114) IL-1 beta antibodies

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          Cite IL-1 beta Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          • Published Figures (1)
          IL-1 beta Antibody in Western Blot (WB)
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          IL-1 beta Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          IL-1 beta Antibody (PA5-119221) in WB

          Western blot analysis of IL-1 beta using IL-1 beta Polyclonal Antibody (Product # PA5-119221) at a dilution of 0.8 µg/mL. Lane 1: RAW264.7 cell lysate; Lane 2: Jurkat cell lysate. Secondary antibody used is HRP-Goat anti-Rabbit IgG polyclonal antibody at a dilution of 0.2 µg/mL. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          IL-1 beta Antibody in Western Blot (WB)
          IL-1 beta Antibody in Western Blot (WB)
          IL-1 beta Antibody in Immunohistochemistry (IHC)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          IL-1 beta Polyclonal Antibody

          Product Details

          PA5-119221

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.01-2 µg/mL
          -

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein Interleukin 1 Beta. The antigen corresponds to amino acid range 118-269 of the target protein.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography, Protein A

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.05% ProClin 300

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2903719

          Target Information

          Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: catabolin; Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; interleukin 1 precursor polypeptide; interleukin 1b; interleukin 1beta; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); preinterleukin 1 beta; Pro interleukin 1 beta; pro-interleukin-1-beta; unnamed protein product

          View more View less

          Gene Aliases: IL-1; Il-1b; IL-1beta; IL1-BETA; IL1beta; IL1F2

          View more View less

          UniProt ID: (Human) P01584, (Mouse) P10749

          View more View less

          Entrez Gene ID: (Human) 3553, (Mouse) 16176

          View more View less

          Function(s)
          cytokine activity interleukin-1 receptor binding integrin binding protein binding protein domain specific binding receptor agonist activity
          Process(es)
          positive regulation of cytokine production microglial cell activation involved in immune response positive regulation of T cell mediated immunity apoptotic process inflammatory response immune response signal transduction cell-cell signaling embryo implantation positive regulation of cell proliferation negative regulation of cell proliferation vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression positive regulation of platelet-derived growth factor receptor signaling pathway positive regulation of epithelial to mesenchymal transition positive regulation of macrophage derived foam cell differentiation negative regulation of glucose transport smooth muscle adaptation cytokine-mediated signaling pathway hyaluronan biosynthetic process monocyte differentiation positive regulation of cell migration positive regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of prostaglandin biosynthetic process positive regulation of fever generation positive regulation of prostaglandin secretion response to lipopolysaccharide negative regulation of interleukin-1 beta production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-2 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of immature T cell proliferation in thymus positive regulation of heterotypic cell-cell adhesion positive regulation of T cell proliferation regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of MAPK cascade positive regulation of MAPK cascade positive regulation of nitric oxide biosynthetic process positive regulation of angiogenesis negative regulation of lipid metabolic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of complement activation positive regulation of JNK cascade negative regulation of insulin receptor signaling pathway positive regulation of protein export from nucleus regulation of defense response to virus by host negative regulation of inflammatory response positive regulation of inflammatory response regulation of neurogenesis negative regulation of neurogenesis regulation of insulin secretion negative regulation of synaptic transmission defense response to Gram-positive bacterium negative regulation of lipid catabolic process positive regulation of lipid catabolic process regulation of nitric-oxide synthase activity positive regulation of membrane protein ectodomain proteolysis positive regulation of protein kinase B signaling positive regulation of glial cell proliferation positive regulation of cell adhesion molecule production negative regulation of adiponectin secretion regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade monocyte aggregation interleukin-1-mediated signaling pathway response to interleukin-1 cellular response to lipopolysaccharide cellular response to mechanical stimulus cellular response to xenobiotic stimulus positive regulation of monocyte chemotactic protein-1 production cellular response to interleukin-17 positive regulation of neuroinflammatory response positive regulation of p38MAPK cascade positive regulation of NIK/NF-kappaB signaling positive regulation of RNA biosynthetic process regulation of establishment of endothelial barrier regulation of secretion by cell negative regulation of gap junction assembly positive regulation of occluding junction disassembly positive regulation of T-helper 1 cell cytokine production negative regulation of interleukin-1-mediated signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of transcription from RNA polymerase II promoter positive regulation of cytosolic calcium ion concentration memory response to carbohydrate negative regulation of gene expression negative regulation of glutamate secretion neutrophil chemotaxis lipopolysaccharide-mediated signaling pathway positive regulation of chemokine production positive regulation of stress-activated MAPK cascade regulation of organic acid transport response to ATP social behavior ectopic germ cell programmed cell death positive regulation of apoptotic process positive regulation of JUN kinase activity positive regulation of neuron apoptotic process negative regulation of neuron differentiation positive regulation of glial cell differentiation positive regulation of transcription from RNA polymerase II promoter astrocyte activation positive regulation of astrocyte differentiation leukocyte migration positive regulation of neutrophil chemotaxis extrinsic apoptotic signaling pathway in absence of ligand negative regulation of secretion by cell negative regulation of branching morphogenesis of a nerve negative regulation of neural precursor cell proliferation
          It has to be done as per old AB suggested Products section.

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