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Invitrogen
ILK Polyclonal Antibody
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FIGURE: 1 / 8
ILK Antibody (PA5-119671) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Integrin linked ILK in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ILK Polyclonal Antibody (Product # PA5-119671) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-119671
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within Human Integrin linked ILK aa 1-290.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Mouse skeletal muscle tissue, rat lung tissue, A431, MG-63, PANC-1, rat skeletal muscle tissue, human kidney tissue, human pancreas tissue, mouse heart tissue.
Subcellular Location: Cell junction, Cell membrane, Cytoplasm.
Target Information
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 59 kDa serine/threonine-protein kinase; beta-integrin-linked kinase; epididymis secretory protein Li 28; ILK; inactive integrin-linked kinase; integrin binding protein kinase; Integrin linked ILK; integrin-linked kinase; integrin-linked kinase-2; integrin-linked protein kinase; unnamed protein product
Gene Aliases: ESTM24; HEL-S-28; ILK-1; ILK-2; P59; p59ILK
UniProt ID: (Human) Q13418, (Rat) Q99J82, (Mouse) O55222
Entrez Gene ID: (Human) 3611, (Rat) 170922, (Mouse) 16202
cell morphogenesis
outflow tract morphogenesis
mitotic spindle organization
cell-matrix adhesion
integrin-mediated signaling pathway
anatomical structure morphogenesis
positive regulation of signal transduction
cell differentiation
positive regulation of BMP signaling pathway
tumor necrosis factor-mediated signaling pathway
substrate adhesion-dependent cell spreading
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of osteoblast differentiation
positive regulation of transcription, DNA-templated
actin filament bundle assembly
actin filament network formation
platelet aggregation
caveola assembly
protein localization to cell cortex
positive regulation of canonical Wnt signaling pathway
positive regulation of substrate adhesion-dependent cell spreading
regulation of cell growth
branching involved in ureteric bud morphogenesis
positive regulation of cell-matrix adhesion
protein phosphorylation
negative regulation of protein kinase activity
positive regulation of cell proliferation
negative regulation of cell proliferation
negative regulation of cardiac muscle cell apoptotic process
fibroblast migration
Schwann cell development
negative regulation of smooth muscle cell migration
peptidyl-serine phosphorylation
nerve development
myelination in peripheral nervous system
positive regulation of cell migration
regulation of actin cytoskeleton organization
negative regulation of apoptotic process
positive regulation of MAP kinase activity
positive regulation of MAPK cascade
protein kinase B signaling
negative regulation of neuron apoptotic process
establishment or maintenance of epithelial cell apical/basal polarity
positive regulation of myoblast differentiation
positive regulation of axon extension
negative regulation of smooth muscle cell proliferation
neuron projection morphogenesis
positive regulation of axonogenesis
positive regulation of dendrite morphogenesis
positive regulation of protein kinase B signaling
fibril organization
negative regulation of neural precursor cell proliferation
positive regulation of cellular senescence
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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