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Invitrogen
GRK3 Polyclonal Antibody
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FIGURE: 1 / 4
GRK3 Antibody (PA5-119939) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of GRK3 in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with GRK3 Polyclonal Antibody (Product # PA5-119939) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI ... View More
Product Details
PA5-119939
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human GRK3 aa 450-688.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Jurkat cell lysate, SHSY5Y cell lysate, HL-60 cell lysate, rat stomachl tissue lysate, MG-63, SiHa, SH-SY5Y.
Subcellular Location: cytosol, plasma membrane.
Target Information
ADRBK2, or adrenergic beta receptor kinase 2 is a serine/threonine kinase that is thought to act as a regulator of receptor function. Overall, the ADRBK2 enzyme, also known as GRK3, has 85% amino acid similarity with ADRBK1, with the protein kinase catalytic domain having 95% similarity. The ADRBK2 mRNA is approximately 8 kilobases with a distribution similar to that of ADRBK1. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adrenergic receptor kinase, beta 2 (G-protein-linked receptor kinase); adrenergic, beta, receptor kinase 2; beta ARK2; Beta-adrenergic receptor kinase 2; Beta-ARK-2; G protein-coupled receptor kinase 3; G-protein-coupled receptor kinase 3; unnamed protein product
Gene Aliases: ADRBK2; BARK2; GRK3
UniProt ID: (Rat) P26819, (Human) P35626
Entrez Gene ID: (Rat) 25372, (Human) 157
protein serine/threonine kinase activity
G-protein coupled receptor kinase activity
ATP binding
D1 dopamine receptor binding
beta-adrenergic receptor kinase activity
nucleotide binding
G-protein coupled receptor binding
protein kinase activity
protein binding
kinase activity
transferase activity
non-receptor serine/threonine protein kinase
positive regulation of protein phosphorylation
desensitization of G-protein coupled receptor protein signaling pathway
protein phosphorylation
intracellular protein transport
signal transduction
multicellular organism aging
receptor internalization
termination of G-protein coupled receptor signaling pathway
inositol phosphate metabolic process
negative regulation of G-protein coupled receptor protein signaling pathway
rhodopsin metabolic process
G-protein coupled receptor signaling pathway
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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