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          • Primary Antibodies ›
          • HMGB1 Antibodies

          Invitrogen

          Acetyl-HMGB1 (Lys29) Polyclonal Antibody

          1 Published Figure
          1 Reference
          View all (59) HMGB1 antibodies

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          Cite Acetyl-HMGB1 (Lys29) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          • Published Figures (1)
          Acetyl-HMGB1 (Lys29) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Acetyl-HMGB1 (Lys29) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          Acetyl-HMGB1 (Lys29) Antibody (PA5-120416) in ICC/IF

          Immunofluorescence analysis of Acetyl-HMGB1 (Lys29) in NIH-3T3 cells. Samples were incubated with Acetyl-HMGB1 (Lys29) Polyclonal antibody (Product # PA5-120416) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Acetyl-HMGB1 (Lys29) Antibody in Immunocytochemistry (ICC/IF)
          Acetyl-HMGB1 (Lys29) Antibody in Immunocytochemistry (ICC/IF)
          Acetyl-HMGB1 (Lys29) Antibody in Immunocytochemistry (ICC/IF)
          Acetyl-HMGB1 (Lys29) Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Acetyl-HMGB1 (Lys29) Polyclonal Antibody

          Product Details

          PA5-120416

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          synthetic peptide
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.67 mg/mL

          Amount

          67 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2913988

          Product Specific Information

          Positive test controls include: 293T. The target is usually found in the following locations: Cell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Endosome, Extracellular side, Nucleus, Peripheral membrane protein, Secreted.

          Target Information

          High-mobility group box 1 protein, previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Ac2-008-like; Amphoterin; Amphoterin antibody; DKFZp686A04236; heparin-binding protein p30; high mobility group 1; high mobility group protein 1; high-mobility group (nonhistone chromosomal) protein 1; HMG-1; HMG3 antibody; hmgb 1; hmgb-1; HMGB1; RP11-550P23.1; Sulfoglucuronyl carbohydrate binding protein; unnamed protein product

          View more View less

          Gene Aliases: Ac2-008; HMG-1; HMG1; HMG3; p30; SBP-1

          View more View less

          UniProt ID: (Human) P09429, (Rat) P63159, (Mouse) P63158

          View more View less

          Entrez Gene ID: (Human) 3146, (Rat) 25459, (Mouse) 15289

          View more View less

          Function(s)
          four-way junction DNA binding bubble DNA binding transcription regulatory region sequence-specific DNA binding lipopolysaccharide binding phosphatidylserine binding DNA binding damaged DNA binding double-stranded DNA binding single-stranded DNA binding transcription coactivator activity transcription corepressor activity RNA binding double-stranded RNA binding single-stranded RNA binding cytokine activity integrin binding protein binding DNA binding, bending calcium-dependent protein kinase regulator activity lyase activity C-X-C chemokine binding protein kinase activator activity chemoattractant activity receptor agonist activity RAGE receptor binding RNA polymerase II sequence-specific DNA binding transcription factor binding DNA polymerase binding supercoiled DNA binding DNA-binding transcription factor binding endodeoxyribonuclease activator activity open form four-way junction DNA binding crossed form four-way junction DNA binding nucleic acid binding bent DNA binding 5S rRNA binding heparin binding peptide binding non-sequence-specific DNA binding, bending protein heterodimerization activity glycolipid binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter cell morphogenesis eye development myeloid dendritic cell activation positive regulation of protein phosphorylation endothelial cell proliferation positive regulation of mesenchymal cell proliferation activation of innate immune response plasmacytoid dendritic cell activation macrophage activation involved in immune response inflammatory response to antigenic stimulus regulation of tolerance induction MyD88-dependent toll-like receptor signaling pathway regulation of T cell mediated immune response to tumor cell DNA repair base-excision repair double-strand break repair double-strand break repair via nonhomologous end joining chromatin organization nucleosome assembly chromatin remodeling chemotaxis inflammatory response immune response positive regulation of cytosolic calcium ion concentration nervous system development memory circadian rhythm negative regulation of DNA replication cell proliferation positive regulation of cell proliferation response to xenobiotic stimulus response to glucose positive regulation of autophagy positive regulation of neuron projection development positive regulation of smooth muscle cell migration negative regulation of RNA polymerase II transcriptional preinitiation complex assembly actin cytoskeleton organization lung development positive regulation of cell migration neuron projection development heterochromatin assembly DNA geometric change positive regulation of mismatch repair response to lipopolysaccharide negative regulation of interferon-gamma production positive regulation of chemokine production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interleukin-1 beta production positive regulation of interleukin-1 production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production response to insulin positive regulation of myeloid cell apoptotic process V(D)J recombination toll-like receptor 2 signaling pathway positive regulation of toll-like receptor 2 signaling pathway toll-like receptor 4 signaling pathway positive regulation of toll-like receptor 4 signaling pathway positive regulation of toll-like receptor 9 signaling pathway response to interferon-gamma T-helper 1 cell activation endothelial cell chemotaxis psychomotor behavior positive regulation of activated T cell proliferation positive regulation of apoptotic process apoptotic cell clearance negative regulation of CD4-positive, alpha-beta T cell differentiation positive regulation of MAPK cascade positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration T-helper 1 cell differentiation positive regulation of innate immune response response to ethanol positive regulation of cell differentiation positive regulation of myeloid cell differentiation positive regulation of myoblast differentiation positive regulation of glycogen catabolic process positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade response to antibiotic male-specific defense response to bacterium induction of positive chemotaxis regulation of DNA metabolic process response to glucocorticoid neuron apoptotic process myoblast proliferation protein-containing complex assembly positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to cytokine stimulus cellular response to interleukin-1 positive regulation of monocyte chemotactic protein-1 production positive regulation of macrophage inflammatory protein 1 alpha production positive regulation of monocyte chemotaxis positive regulation of wound healing neutrophil clearance cellular response to interleukin-7 positive regulation of NIK/NF-kappaB signaling positive regulation of sprouting angiogenesis positive regulation of vascular endothelial cell proliferation positive regulation of chemokine (C-X-C motif) ligand 2 production negative regulation of apoptotic cell clearance regulation of nucleotide-excision repair positive regulation of dendritic cell differentiation positive regulation of endothelial cell proliferation dendritic cell chemotaxis DNA metabolic process DNA topological change DNA recombination signal transduction negative regulation of endothelial cell migration positive regulation of DNA binding positive regulation of viral entry into host cell regulation of viral process positive chemotaxis regulation of hemopoiesis
          It has to be done as per old AB suggested Products section.

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