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Invitrogen
ENPP2 Polyclonal Antibody
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FIGURE: 1 / 1
ENPP2 Antibody (PA5-120818) in WB
Western blot analysis of ENPP2 in extracts of various cell lines. Samples were incubated with ENPP2 Polyclonal antibody (Product # PA5-120818) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-120818
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 564-863 of human ENPP2 (NP_001035181.1).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2.01 mg/mL
Amount
201 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: HepG2, A-549, Mouse kidney. The target is usually found in the following locations: Secreted.
Immunogen sequence: DLGCTCDDKV EPKNKLDELN KRLHTKGSTE ERHLLYGRPA VLYRTRYDIL YHTDFESGYS EIFLMPLWTS YTVSKQAEVS SVPDHLTSCV RPDVRVSPSF SQNCLAYKND KQMSYGFLFP PYLSSSPEAK YDAFLVTNMV PMYPAFKRVW NYFQRVLVKK YASERNGVNV ISGPIFDYDY DGLHDTEDKI KQYVEGSSIP VPTHYYSIIT SCLDFTQPAD KCDGPLSVSS FILPHRPDNE ESCNSSEDES KWVEELMKMH TARVRDIEHL TSLDFFRKTS RSYPEILTLK TYLHTYESEI
Target Information
The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: autotaxin-t; AutotaxinATX; E-NPP 2; ectonucleotide pyrophosphatase/phosphodiesterase 2; ectonucleotide pyrophosphatase/phosphodiesterase family member 2; extracellular lysophospholipase D; FLJ26803; PD-I alpha/autotaxin; ectoenzyme; phosphodiesterase I/nucleotide pyrophosphatase 2; plasma lysophospholipase D; unnamed protein product
Gene Aliases: ATX; ATX-X; AUTOTAXIN; E-NPP 2; LysoPLD; NPP2; Npps2; PD-IALPHA; PDNP2
UniProt ID: (Human) Q13822, (Mouse) Q9R1E6
Entrez Gene ID: (Human) 5168, (Mouse) 18606
nucleic acid binding
phosphodiesterase I activity
lysophospholipase activity
phospholipase D activity
scavenger receptor activity
calcium ion binding
zinc ion binding
hydrolase activity
polysaccharide binding
metal ion binding
alkylglycerophosphoethanolamine phosphodiesterase activity
nucleotide diphosphatase activity
immune response
phospholipid catabolic process
positive regulation of epithelial cell migration
vesicle-mediated transport
sphingolipid catabolic process
regulation of cell migration
phosphatidylcholine catabolic process
positive regulation of lamellipodium morphogenesis
negative regulation of cell-matrix adhesion
phospholipid metabolic process
positive regulation of cell proliferation
estrous cycle
positive regulation of oligodendrocyte differentiation
positive regulation of focal adhesion assembly
cell chemotaxis
positive regulation of substrate adhesion-dependent cell spreading
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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