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Invitrogen
Glycerol kinase Polyclonal Antibody
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FIGURE: 1 / 3
Glycerol kinase Antibody (PA5-120997) in IHC (P)
Immunohistochemistry analysis of Glycerol kinase in paraffin-embedded human esophageal cancer. Samples were incubated with Glycerol kinase Polyclonal antibody (Product # PA5-120997) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
PA5-120997
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 422-559 of Glycerol kinase (GK) (NP_001191948.1).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.04 mg/mL
Amount
104 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: DU145, U-87MG. The target is usually found in the following locations: Cytoplasm, Cytoplasmic side, Mitochondrion outer membrane, Peripheral membrane protein.
Immunogen sequence: GIPLSHLQVD GGMTSNKILM QLQADILYIP VVKPSMPETT ALGAAMAAGA AEGVGVWSLE PEDLSAVTME RFEPQINAEE SEIRYSTWKK AVMKSMGWVT TQSPESGDPS IFCSLPLGFF IVSSMVMLIG ARYISGIP
Target Information
The product of this gene belongs to the FGGY kinase family of proteins and encodes glycerol kinase. Glycerol kinase is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Defects in this gene are the cause of glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been identified.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ASTP; ATP-stimulated glucocorticoid-receptor translocation promoter; ATP-stimulated glucocorticoid-receptor translocaton promoter; ATP:glycerol 3-phosphotransferase; EC 2.7.1.30; GK 3; GK3P; GKl; GKP3; GKTB; Glycerokinase; Glycerokinase 3; Glycerol kinase; Glycerolkinase; unnamed protein product
Gene Aliases: ASTP; D930012N15Rik; GK; GK1; GKD; Gyk
UniProt ID: (Human) P32189, (Rat) Q63060, (Mouse) Q64516
Entrez Gene ID: (Human) 2710, (Rat) 79223, (Mouse) 14933
carbohydrate metabolic process
glycerol metabolic process
glycerol-3-phosphate metabolic process
triglyceride metabolic process
triglyceride biosynthetic process
glycerol catabolic process
glycerol-3-phosphate biosynthetic process
response to cold
response to organic substance
phosphorylation
response to drug
glucose homeostasis
response to ethanol
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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