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Acetyl-Histone H1.4 (Lys45) Polyclonal Antibody
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FIGURE: 1 / 1
Acetyl-Histone H1.4 (Lys45) Antibody (PA5-121379) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Histone H1.4 using Acetyl-Histone H1.4 (Lys45) Polyclonal Antibody (Product # PA5-121379) at a dilution of 1:15 in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30 min at RT. Then primary antibody ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-121379
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthesized peptide derived from Human Histone H1.4 protein (43-55aa)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.16 mg/mL
Amount
16 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody tested in Indirect ELISA
Target Information
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H1 histone family, member 4; H1.4; h1e; histone 1, H1e; Histone cluster 1; histone cluster 1 H1 family member e; histone cluster 1, H1e; histone H1b; histone H1s-4; MGC116819; unnamed protein product
Gene Aliases: dJ221C16.5; H1.4; H1E; H1F4; H1s-4; HIST1H1E; RMNS
UniProt ID: (Human) P10412
Entrez Gene ID: (Human) 3008
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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