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Invitrogen
CRY1 Polyclonal Antibody
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FIGURE: 1 / 5
CRY1 Antibody (PA5-13124) in IHC (P)
Immunohistochemistry analysis of CRY1 in formalin-fixed and paraffin-embedded human cancer tissue. Samples were incubated with CRY1 polyclonal antibody (Product # PA5-13124) which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepato... View More
Product Details
PA5-13124
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 556-586 amino acids from the C-terminal region of human Cry1
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with non-human primate based on sequence homology.
Target Information
Various biochemical, physiological and behavioral processes display circadian rhythms controlled by an internal biological clock. The central "gears" driving this clock appear to be composed of an autoregulatory transcription/post translation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cryptochrome 1 (photolyase-like); cryptochrome circadian clock 1; Cryptochrome-1; unnamed protein product
Gene Aliases: CRY1; DSPD; PHLL1
UniProt ID: (Human) Q16526
Entrez Gene ID: (Human) 1407
nucleotide binding
DNA binding
double-stranded DNA binding
deoxyribodipyrimidine photo-lyase activity
DNA (6-4) photolyase activity
protein binding
photoreceptor activity
blue light photoreceptor activity
ligand-dependent nuclear receptor binding
kinase binding
protein kinase binding
phosphatase binding
histone deacetylase binding
E-box binding
FAD binding
DNA-binding transcription factor binding
negative regulation of transcription from RNA polymerase II promoter
gluconeogenesis
regulation of gluconeogenesis
circadian rhythm
response to light stimulus
blue light signaling pathway
response to activity
lipid storage
negative regulation of protein ubiquitination
positive regulation of protein ubiquitination
response to insulin
circadian regulation of gene expression
response to glucagon
glucose homeostasis
regulation of circadian rhythm
negative regulation of circadian rhythm
signal transduction in response to DNA damage
entrainment of circadian clock by photoperiod
negative regulation of gluconeogenesis
positive regulation of gluconeogenesis
negative regulation of G-protein coupled receptor protein signaling pathway
negative regulation of transcription, DNA-templated
rhythmic process
regulation of DNA damage checkpoint
negative regulation of glucocorticoid receptor signaling pathway
negative regulation of glucocorticoid secretion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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