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Caspase 1 Polyclonal Antibody
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FIGURE: 1 / 5
Caspase 1 Antibody (PA5-140994) in ICC/IF
Immunocytochemistry analysis of Caspase-1 in HepG2 cells (green). Samples were incubated in Caspase-1 Polyclonal antibody (Product # PA5-140994). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-140994
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within human Caspase-1 aa 162-205.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1-b and IL-18. Caspase-1 was initially identified as an IL-1-b-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: caspase 1, apoptosis-related cysteine peptidase; Caspase1; cysteine protease; IL-1 beta-converting enzyme; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; unnamed protein product
Gene Aliases: ICE; IL1BC; P45
UniProt ID: (Human) P29466
Entrez Gene ID: (Human) 834
pattern recognition receptor signaling pathway
proteolysis
apoptotic process
inflammatory response
signal transduction
osmosensory signaling pathway
protein processing
protein autoprocessing
response to lipopolysaccharide
positive regulation of interleukin-1 beta production
positive regulation of interleukin-18 production
defense response to bacterium
regulation of apoptotic process
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of neuron apoptotic process
icosanoid biosynthetic process
regulation of inflammatory response
positive regulation of inflammatory response
protein maturation
defense response to virus
pyroptosis
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
cellular response to interferon-gamma
positive regulation of release of cytochrome c from mitochondria
cytokine precursor processing
signaling receptor ligand precursor processing
AIM2 inflammasome complex assembly
positive regulation of NLRP3 inflammasome complex assembly
positive regulation of tumor necrosis factor-mediated signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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