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Invitrogen
SMARCA2 Polyclonal Antibody
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FIGURE: 1 / 2
SMARCA2 Antibody (PA5-142382) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of SMARCA2 in U251 cells using SMARCA2 Polyclonal Antibody (Product # PA5-142382). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing predominantly nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-142382
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-PSNSQLEIEGNSS.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Additional Information: This antibody is expected to recognise both reported isoforms (NP_003061.3; NP_620614.2).
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:1,000.
Target Information
SMARCA2 encodes a protein that is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Two transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent helicase SMARCA2; BAF190B; brahma homolog; BRG1-associated factor 190B; FLJ36757; global transcription activator homologous sequence; MGC74511; probable global transcription activator SNF2L2; protein brahma homolog; putative global transcription activator SNF2L2; SNF2-alpha; SNF2/SWI2-like protein 2; sucrose nonfermenting 2-like protein 2; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2; unnamed protein product
Gene Aliases: BAF190; BIS; BRM; hBRM; hSNF2a; NCBRS; SAMRCA2; SNF2; SNF2L2; SNF2LA; Sth1p; SWI2
UniProt ID: (Human) P51531
Entrez Gene ID: (Human) 6595
negative regulation of transcription from RNA polymerase II promoter
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
spermatid development
positive regulation of cell proliferation
negative regulation of cell proliferation
regulation of mitotic metaphase/anaphase transition
negative regulation of cell growth
heterochromatin assembly
positive regulation of T cell differentiation
negative regulation of cell differentiation
positive regulation of cell differentiation
positive regulation of myoblast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of G0 to G1 transition
positive regulation of stem cell population maintenance
regulation of G1/S transition of mitotic cell cycle
positive regulation of double-strand break repair
regulation of nucleotide-excision repair
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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