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          • Primary Antibodies ›
          • beta/gamma Catenin Antibodies

          Invitrogen

          Phospho-beta Catenin/gamma Catenin (Tyr142, Tyr133) Polyclonal Antibody

          View all (4) beta/gamma Catenin antibodies

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          Cite Phospho-beta Catenin/gamma Catenin (Tyr142, Tyr133) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          Phospho-beta Catenin/gamma Catenin (Tyr142, Tyr133) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-beta Catenin/gamma Catenin (Tyr142, Tyr133) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Phospho-beta Catenin/gamma Catenin (Tyr142, Tyr133) Antibody (PA5-143664) in ICC/IF

          Immunocytochemistry analysis of Phospho-beta Catenin/gamma Catenin (Tyr142, Tyr133) in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal beta-Catenin antibody and rabbit Phospho-beta Catenin/gamma Catenin (Tyr142, Tyr133) polyclonal antibody (Product # PA5-143664), then the antibodies were detected using appropriate secondary antibodies co... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-beta Catenin/gamma Catenin (Tyr142, Tyr133) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-beta Catenin/gamma Catenin (Tyr142, Tyr133) Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          PA5-143664

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500
          -

          Immunocytochemistry (ICC/IF)

          1:1,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Phospho-beta-Catenin (Tyr-142) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 142 of human beta-Catenin. This peptide sequence has one amino acid difference from a sequence around tyrosine 133 of human gamma-Catenin. These human sequences are highly conserved in rat and mouse beta- and gamma-Catenins.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.125 mg/mL

          Amount

          12.5 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 1mg/mL BSA, 50% glycerol

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2942892

          Product Specific Information

          This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose before affinity purification using phospho-beta-Catenin (Tyr-142) peptide (without carrier). The antibody detects a 92kDa* protein corresponding to the molecular mass of beta-Catenin on SDS-PAGE immunoblots of Hct116 src-transformed cells treated with pervanadate, but not in control cells. Similar results were observed in pervanadate-treated A431 and human endothelial cells.

          Target Information

          beta-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of beta-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between alpha- and beta-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of beta-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in beta-Catenin. The Tyr-654 phosphorylation regulates beta-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of beta-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Bioinformatics

          Protein Aliases: beta-catenin; cadherin associated protein beta 1; catenin (cadherin associated protein), beta 1; catenin (cadherin associated protein), beta 1, 88kDa; catenin (cadherin-associated protein), beta 1, 88kDa; Catenin beta1; CTNNB1; unnamed protein product

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          Gene Aliases: armadillo; Bfc; Catnb; CTNNB; EVR7; Mesc; MRD19; NEDSDV

          View more View less

          UniProt ID: (Human) P35222, (Rat) Q9WU82, (Mouse) Q02248

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          Entrez Gene ID: (Human) 1499, (Rat) 84353, (Mouse) 12387

          View more View less

          Function(s)
          transcription cofactor binding transcription corepressor binding chromatin binding transcription coactivator activity receptor binding protein binding ligand-dependent nuclear receptor binding enzyme binding kinase binding protein kinase binding phosphatase binding protein phosphatase binding estrogen receptor binding ubiquitin protein ligase binding ion channel binding alpha-catenin binding cadherin binding SMAD binding RNA polymerase II sequence-specific DNA binding transcription factor binding I-SMAD binding disordered domain specific binding DNA-binding transcription factor binding histone methyltransferase binding nucleic acid binding ionotropic glutamate receptor binding macromolecular complex binding nitric-oxide synthase binding delta-catenin binding molecular function activator activity
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter protein polyubiquitination embryonic axis specification cell morphogenesis skeletal system development angiogenesis patterning of blood vessels vasculogenesis osteoblast differentiation branching involved in ureteric bud morphogenesis in utero embryonic development gastrulation with mouth forming second endoderm formation cell fate specification cell fate determination endodermal cell fate commitment neuron migration kidney development neural plate development liver development tissue homeostasis vasculature development positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation lens morphogenesis in camera-type eye outflow tract morphogenesis regulation of secondary heart field cardioblast proliferation metanephros morphogenesis negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis regulation of transcription from RNA polymerase II promoter cell adhesion cell-matrix adhesion epidermal growth factor receptor signaling pathway chemical synaptic transmission ectoderm development glial cell fate determination midgut development heart development protein localization cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation response to xenobiotic stimulus response to hormone anterior/posterior axis specification dorsal/ventral axis specification dorsal/ventral pattern formation proximal/distal pattern formation gene expression regulation of gene expression positive regulation of gene expression negative regulation of gene expression regulation of epithelial to mesenchymal transition positive regulation of epithelial to mesenchymal transition positive regulation of heparan sulfate proteoglycan biosynthetic process Schwann cell proliferation response to activity morphogenesis of embryonic epithelium dendrite development negative regulation of angiogenesis layer formation in cerebral cortex hypothalamus development central nervous system vasculogenesis hair cycle process hemopoiesis cell differentiation neuron differentiation T cell differentiation osteoclast differentiation lung development male genitalia development regulation of epithelial cell differentiation positive regulation of epithelial cell differentiation forebrain development midbrain development hindbrain development regulation of centriole-centriole cohesion pancreas development hair follicle morphogenesis regulation of protein ubiquitination regulation of myelination positive regulation of telomere maintenance via telomerase negative regulation of chondrocyte differentiation response to estradiol positive regulation of transcription elongation from RNA polymerase II promoter T cell differentiation in thymus negative regulation of protein sumoylation response to cytokine positive regulation of homotypic cell-cell adhesion cellular response to zinc ion starvation adherens junction organization adherens junction assembly protein localization to cell surface embryonic heart tube development genitalia morphogenesis embryonic forelimb morphogenesis embryonic hindlimb morphogenesis embryonic skeletal limb joint morphogenesis astrocyte-dopaminergic neuron signaling regulation of cell proliferation regulation of T cell proliferation odontogenesis of dentin-containing tooth embryonic digit morphogenesis regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of MAPK cascade negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process skin development response to estrogen cell-cell adhesion mediated by cadherin cellular response to fibroblast growth factor stimulus myoblast differentiation bone resorption regulation of cell differentiation negative regulation of cell differentiation positive regulation of endothelial cell differentiation regulation of osteoblast differentiation positive regulation of osteoblast differentiation regulation of osteoclast differentiation negative regulation of osteoclast differentiation positive regulation of fibroblast growth factor receptor signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of mitotic cell cycle, embryonic response to cadmium ion bone remodeling cell maturation synaptic vesicle transport animal organ development thymus development embryonic organ development oocyte development embryonic foregut morphogenesis positive regulation of skeletal muscle tissue development regulation of smooth muscle cell proliferation negative regulation of oligodendrocyte differentiation negative regulation of neurogenesis synapse organization smooth muscle cell differentiation regulation of timing of anagen regulation of synapse assembly oviduct development canonical Wnt signaling pathway limb development mesenchymal to epithelial transition trachea morphogenesis trachea formation epithelial tube branching involved in lung morphogenesis lung cell differentiation lung-associated mesenchyme development lung induction epithelial cell differentiation involved in prostate gland development positive regulation of epithelial cell proliferation involved in prostate gland development hair follicle placode formation establishment of blood-brain barrier mesenchymal cell proliferation involved in lung development positive regulation of branching involved in lung morphogenesis endothelial tube morphogenesis fungiform papilla formation sympathetic ganglion development cranial ganglion development regulation of centromeric sister chromatid cohesion bicellular tight junction assembly cellular response to mechanical stimulus cellular response to lithium ion cellular response to growth factor stimulus cellular response to indole-3-methanol renal vesicle formation renal inner medulla development renal outer medulla development nephron tubule formation regulation of nephron tubule epithelial cell differentiation mesenchymal stem cell differentiation negative regulation of canonical Wnt signaling pathway regulation of calcium ion import synaptic vesicle clustering cell-cell adhesion positive regulation of odontoblast differentiation negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway cranial skeletal system development midbrain dopaminergic neuron differentiation positive regulation of blood vessel branching epididymis development neuron projection extension cellular response to insulin-like growth factor stimulus embryonic brain development dorsal root ganglion development establishment of blood-retinal barrier regulation of protein localization to cell surface positive regulation of determination of dorsal identity positive regulation of neural precursor cell proliferation negative regulation of apoptotic signaling pathway epithelial to mesenchymal transition stem cell population maintenance hair cell differentiation detection of muscle stretch positive regulation of cell differentiation regulation of angiogenesis positive regulation of angiogenesis regulation of fibroblast proliferation regulation of neurogenesis detection of mechanical stimulus long term synaptic depression ventricular compact myocardium morphogenesis cell-cell junction organization cardiac muscle cell proliferation mesenchyme development cardiac vascular smooth muscle cell differentiation coronary artery morphogenesis epicardium-derived cardiac vascular smooth muscle cell differentiation mesenchyme morphogenesis
          It has to be done as per old AB suggested Products section.

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