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Invitrogen
MUS81 Polyclonal Antibody
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FIGURE: 1 / 1
MUS81 Antibody (PA5-143847) in WB
Western blot analysis of MUS81 using MUS81 antibody (Product # PA5-143847). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human Hela whole cell lysates; Lane 2: human A431 whole cell lysates; Lane 3: human HepG2 whole cell lysates; Lane 4: rat brain tissue lysates; Lane 5: rat PC-12 whole cell lysates; Lane 6: mouse brain tissue lysates; Lane 7: mouse NIH/3T3 whole cell lysates. ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-143847
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human MUS81 recombinant protein (Position: H283-R483).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human A431 whole cell, human HepG2 whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: crossover junction endonuclease MUS81; FLJ21012; FLJ44872 MUS81; MUS81 endonuclease homolog; SLX3 structure-specific endonuclease subunit homolog; unnamed protein product
Gene Aliases: 1200008A18Rik; SLX3
UniProt ID: (Human) Q96NY9, (Mouse) Q91ZJ0, (Rat) Q4KM32
Entrez Gene ID: (Human) 80198, (Mouse) 71711, (Rat) 293678
resolution of meiotic recombination intermediates
telomere maintenance
double-strand break repair via break-induced replication
DNA repair
double-strand break repair
DNA catabolic process
DNA recombination
replication fork processing
intra-S DNA damage checkpoint
osteoblast proliferation
resolution of mitotic recombination intermediates
response to intra-S DNA damage checkpoint signaling
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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