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Invitrogen
PHEX Polyclonal Antibody
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FIGURE: 1 / 4
PHEX Antibody (PA5-143870) in IHC (P)
Immunohistochemistry analysis of PHEX in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with PHEX Polyclonal antibody (Product # PA5-143870) using a dilution of 1 μg/mL overnight at 4°C. Biotinylated goat anti-rabbit IgG was ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-143870
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human PHEX recombinant protein (Position: E145-N718).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL once reconstituted
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: rat ovary tissue, rat kidney tissue, mouse ovary tissue, mouse kidney tissue, human HEK293 whole cell, monkey kidney tissue, rat NRK whole cell, monkey COS-7 whole cell. IHC: human lung cancer tissue, human ovarian cancer tissue. Flow: U87 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Peptidase that cleaves SIBLING (small integrin-binding ligand, N-linked glycoprotein)-derived ASARM peptides, thus regulating their biological activity. Cleaves ASARM peptides between Ser and Glu or Asp residues. Regulates osteogenic cell differentiation and bone mineralization through the cleavage of the MEPE-derived ASARM peptide. Promotes dentin mineralization and renal phosphate reabsorption by cleaving DMP1- and MEPE-derived ASARM peptides. Inhibits the cleavage of MEPE by CTSB/cathepsin B thus preventing MEPE degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: metalloendopeptidase homolog PEX; metalloendopeptidase homolog PEX-like; PEX; PHEX peptidase; phosphate regulating endopeptidase homolog X-linked; phosphate regulating endopeptidase homolog, X-linked; phosphate regulating gene with homologies to endopeptidases on the X chromosome (hypophosphatemia, vitamin D resistant rickets); Phosphate regulating neutral endopeptidase on the X chromosome (X-linked hypophosphatemia XLH); phosphate-regulating neutral endopeptidase; similar to metalloendopeptidase; unnamed protein product; vitamin D-resistant hypophosphatemic rickets protein; X-linked hypophosphatemia protein; X-linked hypophosphatemic rickets candidate gene product
Gene Aliases: HPDR; HPDR1; HYP; HYP1; LXHR; PEX; XLH
UniProt ID: (Human) P78562
Entrez Gene ID: (Human) 5251, (Rat) 25512
skeletal system development
proteolysis
cell-cell signaling
protein processing
lung development
response to vitamin D
protein modification process
bone development
response to growth hormone
cellular response to vitamin D
cellular response to parathyroid hormone stimulus
response to sodium phosphate
response to insulin-like growth factor stimulus
organophosphate metabolic process
bone mineralization
odontogenesis
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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