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Invitrogen
UBE2D1/2/3/4 Polyclonal Antibody
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FIGURE: 1 / 12
UBE2D1/2/3/4 Antibody (PA5-143926) in ICC/IF
Immunocytochemistry analysis of UBE2D1/2/3/4 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in UBE2D1/2/3/4 Polyclonal antibody (Product # PA5-143926) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution an... View More
Product Details
PA5-143926
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human UBE2D1/2/3/4 recombinant protein (Position: D116-M147).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.005mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: rat heart tissue, rat lung tissue, rat liver tissue, human HEK293 whole cell, human Jurkat whole cell, human CACO-2 whole cell. IHC: mouse lung lymph node tissue, human testicular cancer tissue, human bladder cancer tissue, rat lung tissue. ICC/IF: A431 cell. Flow: A431 cell, RAW2647 cell, RH35 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: (E3-independent) E2 ubiquitin-conjugating enzyme D1; (E3-independent) E2 ubiquitin-conjugating enzyme D2; (E3-independent) E2 ubiquitin-conjugating enzyme D3; E2 ubiquitin-conjugating enzyme D1; E2 ubiquitin-conjugating enzyme D2; E2 ubiquitin-conjugating enzyme D2B; E2 ubiquitin-conjugating enzyme D3; E2 ubiquitin-conjugating enzyme D4; E2(17)KB 1; E2(17)KB 2; E2(17)KB 2B; E2(17)KB 3; HBUCE1; p53-regulated ubiquitin-conjugating enzyme 1; PAPase; Phosphoarginine phosphatase; predicted protein of HQ2116; SFT; similar to UBC4/5 in yeast; Stimulator of Fe transport; ubc2e ubiquitin conjugating enzyme; UBC4/5 homolog; UbcH5; UBE2D1/UBE2D3/UBE2D3/UBE2D4; UBE2D3; Ubiquitin carrier protein D1; Ubiquitin carrier protein D2; Ubiquitin carrier protein D2B; Ubiquitin carrier protein D3; Ubiquitin carrier protein D4; ubiquitin conjugating enzyme E2 D4 (putative); ubiquitin conjugating enzyme E2D 1; ubiquitin conjugating enzyme E2D 2; ubiquitin conjugating enzyme E2D 3; ubiquitin conjugating enzyme E2D 4 (putative); Ubiquitin-conjugating enzyme E2 D1; Ubiquitin-conjugating enzyme E2 D2; Ubiquitin-conjugating enzyme E2 D2B; Ubiquitin-conjugating enzyme E2 D3; Ubiquitin-conjugating enzyme E2 D4; Ubiquitin-conjugating enzyme E2(17)KB 1; Ubiquitin-conjugating enzyme E2(17)KB 2; Ubiquitin-conjugating enzyme E2(17)KB 2B; Ubiquitin-conjugating enzyme E2(17)KB 3; Ubiquitin-conjugating enzyme E2-17 kDa 1; Ubiquitin-conjugating enzyme E2-17 kDa 2; Ubiquitin-conjugating enzyme E2-17 kDa 2B; Ubiquitin-conjugating enzyme E2-17 kDa 3; ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2D 1, UBC4/5 homolog; ubiquitin-conjugating enzyme E2D 2; ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme HBUCE1; Ubiquitin-protein ligase D1; Ubiquitin-protein ligase D2; Ubiquitin-protein ligase D2B; Ubiquitin-protein ligase D3; Ubiquitin-protein ligase D4; unnamed protein product
Gene Aliases: 1100001F19Rik; 9430029A22Rik; AA414951; E2(17)KB1; E2(17)KB2; E2(17)KB3; E217kB; HBUCE1; PUBC1; RGD69425; SFT; Ubc2e; UBC4; UBC4/5; UBC5A; UBC5B; UBC5C; UBCH4; UBCH5; UBCH5A; UBCH5B; UBCH5C; UBCH5D; UBE2D1; UBE2D2; Ube2d2b; UBE2D3; UBE2D4
UniProt ID: (Human) P51668, (Human) P62837, (Human) P61077, (Human) Q9Y2X8, (Mouse) P61080, (Rat) D3ZDK2, (Rat) P62839, (Mouse) P61079, (Rat) P61078, (Rat) P70711
Entrez Gene ID: (Human) 7321, (Human) 7322, (Human) 7323, (Human) 51619, (Mouse) 216080, (Rat) 361831, (Rat) 641452, (Mouse) 66105, (Rat) 81920, (Rat) 79435
negative regulation of transcription from RNA polymerase II promoter
protein polyubiquitination
ubiquitin-dependent protein catabolic process
protein ubiquitination
negative regulation of BMP signaling pathway
positive regulation of protein ubiquitination
cellular response to nutrient levels
TORC1 signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
protein K48-linked ubiquitination
positive regulation of protein polyubiquitination
negative regulation of TORC1 signaling
protein modification process
protein autoubiquitination
DNA repair
protein monoubiquitination
apoptotic process
cellular response to DNA damage stimulus
protein K11-linked ubiquitination
protein K6-linked ubiquitination
positive regulation of protein targeting to mitochondrion
protein K29-linked ubiquitination
protein K27-linked ubiquitination
protein K63-linked ubiquitination
metabolic process
biological_process
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