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Invitrogen
GAPDH Polyclonal Antibody
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FIGURE: 1 / 1
GAPDH Antibody (PA5-144252) in WB
Western blot analysis of GAPDH in recombinant protein using a dilution of 3 µg/mL. Samples were incubated with GAPDH polyclonal antibody (Product # PA5-144252). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 36 kDa. observed band size: 36 kDa.
Product Details
PA5-144252
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase protein (2-333AA)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 1.2.1.12; GAPA; GAPA-1; GAPC; GAPC-1; GAPC1; GAPCP-1; GAPCp1; GAPDH; GAPDH-A; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH
Gene Aliases: GAPD; GAPDH; I79_001391
UniProt ID: (Chinese hamster) P17244
Entrez Gene ID: (Chinese hamster) 100736557, (Chinese hamster) 100767862
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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