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Invitrogen
MERTK Polyclonal Antibody
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FIGURE: 1 / 5
MERTK Antibody (PA5-15028) in ICC/IF
Immunocytochemistry analysis of MERTK in U251 cells. Samples were incubated with MERTK polyclonal antibody (Product # PA5-15028) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/m... View More
Product Details
PA5-15028
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
His tag recombinant protein of human MERTK
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
MERTK (cMER) is a tyrosine kinase proto-oncogene and is involved in the retinal pigment epithelium (RPE) phagocytosis pathway, which is implicated in human retinal disease. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: c-mer proto-oncogene tyrosine kinase; MER receptor tyrosine kinase; MGC133349; novel cellular proto-oncogene; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK; sMER; sMERTK; soluble MER; soluble MERTK; STK kinase; Tyrosine-protein kinase Mer; unnamed protein product
Gene Aliases: c-Eyk; c-mer; MER; MERTK; RP38; Tyro12
UniProt ID: (Human) Q12866
Entrez Gene ID: (Human) 10461
natural killer cell differentiation
negative regulation of cytokine production
protein phosphorylation
phagocytosis
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
cell-cell signaling
spermatogenesis
nervous system development
cell migration
platelet activation
secretion by cell
substrate adhesion-dependent cell spreading
apoptotic cell clearance
lymphocyte activation
positive regulation of phagocytosis
negative regulation of lymphocyte activation
establishment of localization in cell
positive regulation of protein kinase B signaling
retina development in camera-type eye
vagina development
neutrophil clearance
negative regulation of leukocyte apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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