Invitrogen

GCK Polyclonal Antibody

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1 Published Figure

1 Reference

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Additional Information:

GCK Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

GCK Antibody (PA5-15072) in ICC/IF

Immunofluorescence analysis of GCK was performed using 70% confluent log phase PANC-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GCK Polyclonal Antibody (Product # PA5-15072) at 1:50 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) ... View More

GCK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

GCK Antibody — Figure 6 HK activity is significantly inhibited in vitro by POA and BA. ( A ) HK1 activity is reduced significantly by 0.1 mM POA ( n = 6, P < 0.0001), but not changed significantly by 0.05% BA ( n = 4, P > 0.13) as compared with control ( n = 6). ( B ) HK2 activity is reduced significantly by 0.1 mM POA ( n = 9, P < 0.0001), but not affected by 0.05% BA ( n = 4, P > 0.3) as compared with control ( n = 13). ( C ) HK4 activity is reduced significantly by 0.05% BA ( n = 8, P < 0.0001), but not affected by 0.1 mM POA ( n = 4, P > 0.8) as compared with control ( n = 6). ( D ) Western blot analysis of the expression levels of HK1, HK2, and HK4 in PACs (representative case, repeated 3 times with similar results). *** P < 0.001, 1-way ANOVA. {TM}

Immunogen image

Immunogen Image GCK Polyclonal Antibody

Product Details

PA5-15072

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

1:10-1:50

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GCK

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

400 µg

Purification

Ammonium sulfate precipitation, Size-exclusion - Dialysis

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Ambient (domestic); Wet ice (international)

AB_2247319

Target Information

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ATP:D-hexose 6-phosphotransferase; GC kinase; glucokinase (EC 2.7.1.1); glucokinase (hexokinase 4); hexokinase D, pancreatic isozyme; hexokinase type IV; hexokinase-D; HK IV; HK4; MEKKK 2; unnamed protein product

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Gene Aliases: FGQTL3; GK; GLK; Gls006; GLUKA; HHF3; HK4; HKIV; Hlb62; HXKP; LGLK; MODY2; PNDM1; RNGK2

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UniProt ID: (Human) P35557, (Mouse) P52792, (Rat) P17712

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Entrez Gene ID: (Human) 2645, (Mouse) 103988, (Rat) 24385

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Function(s)

glucokinase activity hexokinase activity protein binding ATP binding glucose binding fructokinase activity mannokinase activity glucose sensor activity magnesium ion binding protein phosphatase binding ADP binding

Process(es)

cellular glucose homeostasis carbohydrate metabolic process glycogen biosynthetic process fructose 6-phosphate metabolic process fructose 2,6-bisphosphate metabolic process glucose metabolic process glucose catabolic process mannose metabolic process glycolytic process purine nucleotide metabolic process NADP metabolic process positive regulation of cytosolic calcium ion concentration response to glucose hexose metabolic process positive regulation of insulin secretion negative regulation of epinephrine secretion cellular response to insulin stimulus cellular response to glucose starvation positive regulation of phosphorylation glucose homeostasis regulation of potassium ion transport cellular response to leptin stimulus negative regulation of gluconeogenesis positive regulation of glycogen biosynthetic process positive regulation of glycolytic process nicotinamide nucleotide metabolic process carbohydrate phosphorylation regulation of insulin secretion glucose 6-phosphate metabolic process lipid homeostasis calcium ion import regulation of glucose mediated signaling pathway regulation of glycolytic process canonical glycolysis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GCK Polyclonal Antibody

GCK Antibody (PA5-15072) in ICC/IF

Product Details

PA5-15072

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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