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Invitrogen
PERK Polyclonal Antibody
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FIGURE: 1 / 6
PERK Antibody (PA5-15305) in IHC (P)
Immunohistochemistry analysis of PERK in formalin-fixed and paraffin-embedded human hepatocarcinoma tissue. Samples were incubated with PERK polyclonal antibody (Product # PA5-15305) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Details
PA5-15305
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 148-175 amino acids from the N-terminal region of human PERK
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Size-exclusion - Dialysis, Ammonium sulfate precipitation
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
EIF2AK3 (PERK) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK3, signaling is initiated by misfolded proteins in the ER. Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS), also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2.7.11.1; eIF-2 protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 3; HsPEK; OTTHUMP00000207187; OTTHUMP00000207188; pancreatic eIF-2 alpha kinase; pancreatic eIF-2alpha kinase; Pancreatic eIF2-alpha kinase; PKR-like ER kinase; PRKR-like endoplasmic reticulum kinase; Protein tyrosine kinase EIF2AK3; unnamed protein product
Gene Aliases: EIF2AK3; PEK; PERK; WRS
UniProt ID: (Human) Q9NZJ5
Entrez Gene ID: (Human) 9451
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
eukaryotic translation initiation factor 2alpha kinase activity
protein tyrosine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
enzyme binding
protein phosphatase binding
identical protein binding
translation regulator activity
misfolded protein binding
Hsp90 protein binding
protein serine kinase activity
skeletal system development
ossification
angiogenesis
chondrocyte development
translational initiation
regulation of translation
regulation of translational initiation
response to oxidative stress
ER overload response
response to unfolded protein
endoplasmic reticulum organization
positive regulation of vascular endothelial growth factor production
positive regulation of gene expression
negative regulation of translation
calcium-mediated signaling
bone mineralization
endoplasmic reticulum unfolded protein response
endocrine pancreas development
negative regulation of myelination
negative regulation of translation in response to stress
negative regulation of translational initiation in response to stress
cellular response to amino acid starvation
response to endoplasmic reticulum stress
regulation of translation initiation in response to endoplasmic reticulum stress
PERK-mediated unfolded protein response
cellular response to glucose starvation
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of transcription from RNA polymerase I promoter
negative regulation of translational initiation
insulin-like growth factor receptor signaling pathway
cellular response to cold
mitochondrial respiratory chain supercomplex assembly
positive regulation of protein localization to nucleus
regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
response to manganese-induced endoplasmic reticulum stress
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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