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Invitrogen
ATG3 Polyclonal Antibody
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FIGURE: 1 / 5
ATG3 Antibody (PA5-17018) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of HT-29 (Lane 1), HeLa (Lane 2), C2C12 (Lane 3), HEK 293 (Lane 4), MCF7 (Lane 5), U-87 MG (Lane 6), A-431 (Lane 7) and U-2 OS (Lane 8). The blot was probed with Anti-ATG3 Polyclonal Antibody (Product # PA5-17018, 1:1,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). A 40 kDa band corresponding to ATG3 was observed across the cell li... View More
Product Details
PA5-17018
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Human,
Mouse,
Non-human primate,
Rat,
Xenopus
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues near the amino terminus of Atg3
Conjugate
Form
Liquid
Concentration
29 µg/mL
Amount
2.9 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Autophagy is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: APG3 autophagy 3-like; APG3-like; Apg3p; ATG3 autophagy related 3 homolog; autophagy-related 3; Autophagy-related protein 3; DKFZp564M1178; FLJ22125; MGC15201; Preconditioning-inducible gene 1 protein; Protein PC3-96; Ubiquitin-like-conjugating enzyme ATG3; unnamed protein product
Gene Aliases: 2610016C12Rik; APG3; APG3-LIKE; APG3L; ATG3; Atg3l; hApg3; PC3-96; PIG-1; Pig1; XELAEV_18014721mg
UniProt ID: (Bovine) Q0VCL3, (Human) Q9NT62, (Rat) Q6AZ50, (Mouse) Q9CPX6, (Xenopus) Q6GQE7
Entrez Gene ID: (Bovine) 508571, (Chicken) 418369, (Human) 64422, (Dog) 478564, (Rat) 171415, (Mouse) 67841, (Xenopus) 443885
Atg8 ligase activity
Atg12 transferase activity
enzyme binding
protein binding
transferase activity
ubiquitin-like protein transferase activity
Atg8-family conjugating enzyme activity
molecular_function
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
autophagosome assembly
protein transport
protein ubiquitination
mitochondrial fragmentation involved in apoptotic process
negative regulation of phagocytosis
regulation of cilium assembly
mitophagy
protein targeting to membrane
autophagy
macroautophagy
nucleophagy
glycophagy
cellular protein modification process
transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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