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Invitrogen
HSP40 Polyclonal Antibody
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FIGURE: 1 / 8
HSP40 Antibody (PA5-17382) in ICC/IF
Immunofluorescence analysis of HSP40 was performed using 70% confluent log phase HeLa cells heat shocked at 43°C for 1 hr. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSP40 Polyclonal Antibody (Product # PA5-17382) at 1:400 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® ... View More
Product Details
PA5-17382
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to Glu300 of human HSP40/Hdj1
Conjugate
Form
Liquid
Concentration
35 µg/mL
Amount
3.5 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other HSPs.
Target Information
TCF-9 (transcription factor 9), also known as C2orf3 (chromosome 2 open reading frame 3), GCF (GC-rich sequence DNA-binding factor) or DNABF, is a 781 amino acid nuclear protein that belongs to the GCF family. Widely expressed, GCF binds the GC-rich sequences of beta-Actin, EGFR and calcium-dependent protease (CANP) promoters. GCF contains multiple phosphoserine and phosphothreonine residues, and two GCF isoforms are produced due to alternative splicing events. GCF is considered a candidate for susceptibility to dyslexia (DYX3) as both genes reside in close proximity on human chromosome 2. Chromosome 2 is the second largest human chromosome and consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJB1; hDj-1; Heat shock 40 kDa protein 1; Heat shock protein; HSP; HSP 40; HSP40; Human DnaJ protein 1; NDAJB1; radial spoke 16 homolog B; unnamed protein product
Gene Aliases: 0610007I11Rik; DjB1; DNAJ1; DNAJB1; HDJ1; Hsp40; HSPF1; RSPH16B; Sis1
UniProt ID: (Human) P25685, (Mouse) Q9QYJ3
Entrez Gene ID: (Human) 3337, (Rat) 361384, (Mouse) 81489
negative regulation of transcription from RNA polymerase II promoter
protein folding
response to unfolded protein
forebrain development
cellular response to heat
negative regulation of inclusion body assembly
regulation of cellular response to heat
positive regulation of protein folding
positive regulation of ATPase activity
chaperone mediated protein folding requiring cofactor
chaperone cofactor-dependent protein refolding
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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