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Invitrogen
HP1 alpha Polyclonal Antibody
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FIGURE: 1 / 6
HP1 alpha Antibody (PA5-17441) in ICC/IF
Immunofluorescence analysis of Chromobox protein homolog 5 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with HP1 alpha Polyclonal Antibody (Product # PA5-17441) at 1:100 dilution in 0.1% BSA, incubated... View More
Product Details
PA5-17441
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the carboxy terminus of human HP1alpha
Conjugate
Form
Liquid
Concentration
120 µg/mL
Amount
12 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with HP1 beta or HP1 gamma proteins.
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 25 kda chromosomal autoantigen; Antigen p25; CBX5; Cbx5 antibody; chromobox homolog 5; chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox protein homolog 5; epididymis luminal protein 25; heterochromatin protein 1 alpha; Heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha; HGNC:1555; HP-1 alpha antibody; HP1 alpha; HP1 alpha homolog; HP1-ALPHA; HP1Hs alpha; HP1Hs-alpha; mHP1(alpha); unnamed protein product
Gene Aliases: 2610029O15Rik; C75991; CBX5; HEL25; HP1; HP1A; HP1alpha
UniProt ID: (Human) P45973, (Mouse) Q61686
Entrez Gene ID: (Human) 23468, (Rat) 300266, (Mouse) 12419
chromatin binding
protein binding
protein binding, bridging
identical protein binding
histone deacetylase binding
ribonucleoprotein complex binding
macromolecular complex binding
histone H3K9me2/3 reader activity
DNA-binding transcription factor binding
histone reader activity
molecular_function
methylated histone binding
protein homodimerization activity
repressing transcription factor binding
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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