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FIGURE: 1 / 10
Calpain 2 Antibody (PA5-17494) in WB
Western blot was performed using Anti-Calpain 2 Polyclonal Antibody (Product # PA5-17494) and a 76 kDa band corresponding to Calpain 2 was observed in C2C12, DU 145, NIH/3T3, A-431 and PC-3, but not in Jurkat and Daudi which are reported to be negative. Whole cell lysates (30 µg lysate) of Jurkat (Lane 1), C2C12 (Lane 2), DU 145 (Lane 3), NIH/3T3 (Lane 4), A-431 (Lane 5), PC-3 (Lane 6) and Daudi (Lane 7) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-17494
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the human sequence of calpain 2 (large subunit)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
54 µg/mL
Amount
5.4 µg
Purification
Antigen affinity chromatography, Protein A
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases. Mu-calpain has a micromolar sensitivity (thus the mu-) as compared to the millimolar calcium sensitivity of m-calpain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 80 kDa M-calpain subunit; Ca2-activated neutral protease subunit; calcium-activated neutral proteinase 2; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; calpain II; calpain M-type; calpain, large polypeptide L2; calpain-2 large subunit; CANP 2; cysteine proteinase; M-calpain; m-calpain 80 kDa large subunit; m-calpain large subunit; millimolar-calpain; unnamed protein product
Gene Aliases: CALP80; CANP2; CANPL2; CANPml; Capa-2; Capa2; m-calpain; m-calpin; mCANP
UniProt ID: (Human) P17655, (Rat) Q07009, (Mouse) O08529
Entrez Gene ID: (Human) 824, (Rat) 29154, (Mouse) 12334
response to hypoxia
proteolysis
female pregnancy
response to mechanical stimulus
positive regulation of cardiac muscle cell apoptotic process
protein autoprocessing
protein catabolic process
regulation of interleukin-6 production
cellular response to interferon-beta
response to hydrogen peroxide
behavioral response to pain
synaptic vesicle endocytosis
regulation of cytoskeleton organization
negative regulation of focal adhesion assembly
cellular response to lipopolysaccharide
cellular response to amino acid stimulus
vascular endothelial cell response to laminar fluid shear stress
vascular endothelial cell response to oscillatory fluid shear stress
protein catabolic process at postsynapse
positive regulation of myoblast fusion
positive regulation of phosphatidylcholine biosynthetic process
blastocyst development
myoblast fusion
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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