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          • Primary Antibodies ›
          • EGFR Antibodies

          Invitrogen

          Phospho-EGFR (Tyr1148) Polyclonal Antibody

          Advanced Verification
          View all (295) EGFR antibodies

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          Cite Phospho-EGFR (Tyr1148) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          • Advanced Verification (1)
          Phospho-EGFR (Tyr1148) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-EGFR (Tyr1148) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          Phospho-EGFR (Tyr1148) Antibody (PA5-17640) in ICC/IF

          Immunofluorescence analysis of Phospho-EGFR (Tyr1148) was performed using 90% confluent log phase A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-EGFR (Tyr1148) Rabbit Polyclonal Antibody (Pro... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-EGFR (Tyr1148) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-EGFR (Tyr1148) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-EGFR (Tyr1148) Antibody
          Phospho-EGFR (Tyr1148) Polyclonal Antibody

          Product Details

          PA5-17640

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200
          -

          Immunocytochemistry (ICC/IF)

          1:100
          -
          Product Specifications

          Species Reactivity

          Human, Non-human primate, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic phosphopeptide corresponding to residues surrounding pTyr1148 of human EGF receptor
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10982211

          Product Specific Information

          It is not recommended to aliquot this antibody.

          This antibody is not cross-reactive with other tyrosine-phosphorylated ErbB family members.

          Target Information

          EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; contains extracellular domain; EC 2.7.10.1; EGFR vIII; EGFR-related peptide; Epidermal growth factor receptor; Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor tyrosine kinase domain; epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); erb-b2 receptor tyrosine kinase 1; GIG40; kinase EGFR; membrane protein; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; unnamed protein product; Urogastrone

          View more View less

          Gene Aliases: EGFR; ERBB; ErbB-1; ERBB1; ERRP; HER1; mENA; NISBD2; NNCIS; PIG61

          View more View less

          UniProt ID: (Human) P00533

          View more View less

          Entrez Gene ID: (Human) 1956, (Rat) 24329

          View more View less

          Function(s)
          nucleotide binding virus receptor activity chromatin binding double-stranded DNA binding protein kinase activity MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity protein binding ATP binding kinase activity transferase activity enzyme binding kinase binding protein phosphatase binding protein tyrosine kinase activator activity ubiquitin protein ligase binding identical protein binding cadherin binding epidermal growth factor binding actin filament binding ATPase binding glycoprotein binding receptor signaling protein tyrosine kinase activity signal transducer activity receptor binding integrin binding protein kinase binding nitric-oxide synthase regulator activity protein heterodimerization activity transmembrane signal receptor
          Process(es)
          MAPK cascade cell morphogenesis ossification embryonic placenta development positive regulation of protein phosphorylation hair follicle development signal transduction cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway epidermal growth factor receptor signaling pathway salivary gland morphogenesis learning or memory cell proliferation positive regulation of cell proliferation epidermis development cerebral cortex cell migration neuron differentiation positive regulation of cell growth positive regulation of cell migration positive regulation of peptidyl-serine phosphorylation ERBB2-EGFR signaling pathway negative regulation of epidermal growth factor receptor signaling pathway regulation of cell proliferation negative regulation of protein catabolic process positive regulation of phosphorylation negative regulation of apoptotic process positive regulation of MAPK cascade protein kinase B signaling positive regulation of DNA repair positive regulation of DNA replication positive regulation of transcription from RNA polymerase II promoter viral entry into host cell positive regulation of fibroblast proliferation digestive tract morphogenesis epithelial cell proliferation positive regulation of epithelial cell proliferation regulation of peptidyl-tyrosine phosphorylation protein insertion into membrane positive regulation of protein kinase B signaling morphogenesis of an epithelial fold eyelid development in camera-type eye response to UV-A positive regulation of ERK1 and ERK2 cascade cellular response to amino acid stimulus cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus cellular response to estradiol stimulus positive regulation of protein kinase C signaling positive regulation of canonical Wnt signaling pathway cell-cell adhesion positive regulation of G1/S transition of mitotic cell cycle positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of protein localization to plasma membrane negative regulation of cardiocyte differentiation activation of MAPKK activity liver development translation response to osmotic stress response to oxidative stress circadian rhythm magnesium ion homeostasis response to organic cyclic compound diterpenoid metabolic process single organismal cell-cell adhesion peptidyl-tyrosine phosphorylation signal transduction by protein phosphorylation lung development positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle response to estradiol positive regulation of superoxide anion generation response to cobalamin response to hydroxyisoflavone response to lipid positive regulation of catenin import into nucleus intracellular signal transduction cellular response to drug ovulation cycle hydrogen peroxide metabolic process positive regulation of MAP kinase activity tongue development positive regulation of nitric oxide biosynthetic process positive regulation of vasoconstriction positive regulation of vasodilation negative regulation of mitotic cell cycle protein autophosphorylation astrocyte activation positive regulation of smooth muscle cell proliferation neuron projection morphogenesis positive regulation of inflammatory response regulation of nitric-oxide synthase activity response to calcium ion positive regulation of synaptic transmission, glutamatergic cellular response to mechanical stimulus cellular response to dexamethasone stimulus
          It has to be done as per old AB suggested Products section.

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