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Invitrogen
Phospho-CHK2 (Thr68) Polyclonal Antibody
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FIGURE: 1 / 5
Phospho-CHK2 (Thr68) Antibody (PA5-17818) in ICC/IF
Immunofluorescent analysis of Phospho-Chk2 pThr68 (green) in HeLa cells either left untreated (left panel) or treated with 25 µg/mL anisomycin for 30 minutes (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-Chk2 pThr68 p... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-17818
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding pThr68 of human Chk2
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
99 µg/mL
Amount
9.9 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with Chk2 phosphorylated at other sites.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
Target Information
CHEK2 (CHK2) is a serine/threonine kinase and a component of the DNA damage checkpoint pathway. A mutation in CHK2 has been linked to cancer. CHK2 is activated by DNA damage and phosphorylates several modulators of cell cycle control including tumor suppressor proteins. In addition, Chk2 can phosphorylate BRCA1, allowing BRCA1 to restore survival after DNA damage. Chk2 is a putative tumor suppressor protein, with mutations in to the Chk2 gene linked to Li-Fraumeni syndrome, a familiar cancer usually associated with inherited mutations in TP53. Also, mutations in CHK2 are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cds1; cds1 homolog; Checkpoint like protein CHK2; checkpoint-like protein CHK2; CHEK2; Chk2 (phospho T68); Chk2 (pThr68); CHK2 checkpoint homolog; CHK2 checkpoint homolog (S. pombe); Chk2 phospho; Chk2 phospho T68; Chk2-del2-12; Chk2-del2-3; Chk2-del4; Chk2-del7; Chk2-del9; Chk2-del9-12; Chk2-insX; Chk2-insZ; Chk2-iso1; Chk2-iso2; Chk2-sub3; contains FHA domain; del9; EC 2.7.11.1; HuCds 1; kinase Chk2; OTTHUMP00000199044; OTTHUMP00000199045; OTTHUMP00000199064; OTTHUMP00000199115; OTTHUMP00000199116; phospho Chk2 (T68); phospho Thr68 Chk2; RP11-436C9.1; Serine/threonine protein kinase Chk2; unnamed protein product
Gene Aliases: CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53; TPDS4
UniProt ID: (Human) O96017
Entrez Gene ID: (Human) 11200
DNA damage checkpoint
G2/M transition of mitotic cell cycle
double-strand break repair
regulation of transcription, DNA-templated
protein phosphorylation
cellular response to DNA damage stimulus
response to oxidative stress
intrinsic apoptotic signaling pathway in response to DNA damage
protein catabolic process
DNA damage response, signal transduction by p53 class mediator
intra-S DNA damage checkpoint
cellular response to stress
regulation of protein catabolic process
response to starvation
signal transduction in response to DNA damage
mitotic DNA damage checkpoint
positive regulation of transcription, DNA-templated
protein autophosphorylation
protein stabilization
mitotic spindle assembly
replicative senescence
regulation of signal transduction by p53 class mediator
regulation of autophagosome assembly
positive regulation of autophagosome assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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