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Arginase 1 Polyclonal Antibody
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FIGURE: 1 / 6
Arginase 1 Antibody (PA5-18684) in ICC/IF
Immunocytochemistry analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Samples were incubated with Arginase 1 polyclonal antibody (Product # PA5-18684) using a dilution of 10 µg/mL for 1 hr followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-18684
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (NHKPETDYLKPPK) corresponding to the C-terminus amino acids of Arg1 (aa 311-323).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: A-I; AI type I arginase; Arg1; Arginase; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase1; HGNC:663; Liver Arginase; liver arginase (E.C. 3.5.3.1); liver-type arginase; Type 1 Arginase; type I arginase; unnamed protein product
Gene Aliases: AI; Arg-1; PGIF
UniProt ID: (Mouse) Q61176, (Rat) P07824
Entrez Gene ID: (Mouse) 11846, (Rat) 29221
urea cycle
positive regulation of endothelial cell proliferation
arginine metabolic process
arginine catabolic process
response to nematode
response to selenium ion
collagen biosynthetic process
regulation of cell proliferation
negative regulation of T cell proliferation
defense response to protozoan
negative regulation of activated T cell proliferation
negative regulation of interferon-gamma-mediated signaling pathway
neutrophil mediated killing of fungus
positive regulation of neutrophil mediated killing of fungus
regulation of L-arginine import across plasma membrane
negative regulation of T-helper 2 cell cytokine production
liver development
female pregnancy
response to xenobiotic stimulus
response to herbicide
response to manganese ion
response to zinc ion
response to amine
lung development
response to lipopolysaccharide
response to vitamin A
response to vitamin E
response to amino acid
response to peptide hormone
response to cadmium ion
response to steroid hormone
response to axon injury
response to methylmercury
mammary gland involution
maternal process involved in female pregnancy
cellular response to hydrogen peroxide
cellular response to lipopolysaccharide
cellular response to interleukin-4
cellular response to glucagon stimulus
cellular response to dexamethasone stimulus
cellular response to transforming growth factor beta stimulus
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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