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Antibody Testing Data (1)

FIGURE: 1 / 1

PRMT5 Antibody (PA5-19172) in WB

Western blot analysis of PRMT5 using PRMT5 Polyclonal Antibody (Product # PA5-19172) (2 µg/mL) in staining of Jurkat lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image PRMT5 Polyclonal Antibody

Product Details

PA5-19172

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (RACREKDRDPEAQ) corresponding to the internal amino acids of PRMT5 (aa 485-498).

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

100 µg

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Ambient (domestic); Wet ice (international)

AB_10989302

Product Specific Information

This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

Target Information

PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3; such methylation is required for the assembly and biogenesis of snRNP core particles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 72 kDa ICln-binding protein; EC 2.1.1.125; histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; homolog of fission yeast Skb1; jak-binding protein 1; JBP1; homodimer; shk1 kinase-binding protein 1 homolog; SKB1; SKB1 homolog; unnamed protein product

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Gene Aliases: HRMT1L5; HSL7; IBP72; JBP1; SKB1; SKB1Hs

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UniProt ID: (Human) O14744

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Entrez Gene ID: (Human) 10419

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Function(s)

p53 binding transcription corepressor activity protein binding methyltransferase activity methyl-CpG binding protein-arginine N-methyltransferase activity protein-arginine omega-N symmetric methyltransferase activity histone methyltransferase activity identical protein binding ribonucleoprotein complex binding histone methyltransferase activity (H4-R3 specific) macromolecular complex binding protein heterodimerization activity E-box binding histone H3 methyltransferase activity

Process(es)

spliceosomal snRNP assembly chromatin remodeling DNA-templated transcription, termination regulation of transcription, DNA-templated regulation of mitotic nuclear division regulation of gene expression circadian regulation of gene expression peptidyl-arginine N-methylation endothelial cell activation hypermethylation of CpG island negative regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of mRNA splicing, via spliceosome positive regulation of oligodendrocyte differentiation regulation of ERK1 and ERK2 cascade cellular response to growth factor stimulus Golgi ribbon formation liver regeneration negative regulation of cGAS/STING signaling pathway regulation of signal transduction by p53 class mediator positive regulation of adenylate cyclase-inhibiting dopamine receptor signaling pathway positive regulation of rRNA processing

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PRMT5 Polyclonal Antibody

PRMT5 Antibody (PA5-19172) in WB

Product Details

PA5-19172

Western Blot (WB)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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