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Invitrogen
ECSIT Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-20082
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A peptide corresponding to 14 amino acids near the C-terminus of human ECSIT.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Ion-exchange chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is human heart tissue lysate.
PA5-20082 can be used with blocking peptide PEP-0200.
Target Information
Activation of NF-kappa-B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF)-6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ECSIT homolog; ECSIT signalling integrator; likely ortholog of mouse signaling intermediate in Toll pathway evolutionarily conserved; putative intermediate in the Toll/IL-1 signal transduction pathway; signaling intermediate in Toll pathway-evolutionarily conserved; SITPEC; TRAF6 binding protein
Gene Aliases: SITPEC
UniProt ID: (Human) Q9BQ95, (Mouse) Q9QZH6
Entrez Gene ID: (Human) 51295, (Mouse) 26940
transmembrane receptor protein serine/threonine kinase signaling pathway
mitochondrial respiratory chain complex I assembly
toll-like receptor 4 signaling pathway
innate immune response
mesoderm formation
regulation of transcription from RNA polymerase II promoter
BMP signaling pathway
NIK/NF-kappaB signaling
regulation of oxidoreductase activity
regulation of protein complex stability
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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