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Invitrogen
ARC Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-20305
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 14 amino acid peptide from near the carboxy terminus of human Nop30.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is mouse skeletal muscle tissue lysate.
PA5-20305 can be used with blocking peptide PEP-0399.
Target Information
ARC (also known as NOL3 - Nucleolar protein 3) has two isoforms. Isoform 1 is involved in RNA splicing. Isoform 2 functions as an apoptosis repressor that blocks multiple modes of cell death. ARC inhibits extrinsic apoptotic pathways two different ways. The first is by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly. The second way is by interacting with CASP8 in a mitochondria localization-and phosphorylation-dependent manner, which in turn limits the amount of soluble CASP8 avaliable for DISC-mediated activation. ARC also inhibits intrinsic apoptotic pathways in response to a wide range of stresses through interactions with BAX. This results in BAX inactivation and prevents mitochondrial dysfunction and release of pro-apoptotic factors. Mutations affecting the gene can results in myoclonus, familiam cortical.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: apoptosis repressor with CARD; ARC; muscle specific protein; muscle-enriched cytoplasmic protein; Nop 30; nucleolar protein 3 (apoptosis repressor with CARD domain); nucleolar protein of 30 kDa; unnamed protein product
Gene Aliases: ARC; B430311C09Rik; FCM; MYC; MYOCL1; MYP; NOP; NOP30
UniProt ID: (Human) O60936, (Mouse) Q9D1X0, (Rat) Q62881
Entrez Gene ID: (Human) 8996, (Mouse) 78688, (Rat) 85383
RNA binding
receptor binding
death receptor binding
calcium ion binding
protein binding
death effector domain binding
identical protein binding
cysteine-type endopeptidase inhibitor activity involved in apoptotic process
caspase binding
cysteine-type endopeptidase inhibitor activity
kinase binding
phosphatase binding
response to hypoxia
blood vessel remodeling
response to ischemia
mRNA splice site selection
negative regulation of signal transduction
regulation of gene expression
cardiac muscle cell apoptotic process
negative regulation of striated muscle cell apoptotic process
negative regulation of cardiac muscle cell apoptotic process
negative regulation of tumor necrosis factor-mediated signaling pathway
regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
negative regulation of muscle atrophy
release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
response to injury involved in regulation of muscle adaptation
regulation of apoptotic process
negative regulation of apoptotic process
myoblast differentiation
smooth muscle cell proliferation
protein oligomerization
negative regulation of cytosolic calcium ion concentration
negative regulation of mitochondrial calcium ion concentration
negative regulation of programmed necrotic cell death
cellular response to hypoxia
negative regulation of release of cytochrome c from mitochondria
intrinsic apoptotic signaling pathway
regulation of NIK/NF-kappaB signaling
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of mitochondrial membrane permeability involved in apoptotic process
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
regulation of intracellular signal transduction
negative regulation of death-inducing signaling complex assembly
negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
negative regulation of establishment of protein localization to mitochondrion
negative regulation of extrinsic apoptotic signaling pathway
negative regulation of intrinsic apoptotic signaling pathway
cellular response to hydrogen peroxide
RNA splicing
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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