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          • Primary Antibodies ›
          • PTEN Antibodies

          Invitrogen

          PTEN Polyclonal Antibody

          2 References
          View all (23) PTEN antibodies

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          Cite PTEN Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          PTEN Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          PTEN Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          PTEN Antibody (PA5-20418) in ICC/IF

          Immunofluorescence of PTEN in P815 cells with PTEN Polyclonal Antibody (Product # PA5-20418) at 2 µg/mL. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          PTEN Antibody in Immunocytochemistry (ICC/IF)
          PTEN Antibody in Western Blot (WB)
          PTEN Polyclonal Antibody

          Product Details

          PA5-20418

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -

          Miscellaneous PubMed (Misc)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A 15 amino acid peptide from near the amino terminus of human PTEN.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS

          Contains

          0.02% sodium azide

          Storage conditions

          Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_11152102

          Product Specific Information

          A suggested positive control is P815 cell lysate.

          PA5-20418 can be used with blocking peptide PEP-0535.

          Target Information

          PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The PTEN gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. PTEN negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. PTEN plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Also, PTEN may be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form of PTEN induces less tumor suppressive ability.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Inositol polyphosphate 3-phosphatase; mitochondrial phosphatase and tensin protein alpha; mitochondrial PTENalpha; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; Mutated in multiple advanced cancers 1; N-terminal extended PTEN isoform; uses an alternative ATT initiation codon in PTEN 5'UTR; phosphatidylinositol-3,4,5-trisphosphate3-phosphatase; phosphatase and tensin; Phosphatase and tensin homolog; phosphatase and tensin-like protein; phosphatidylinositol 3; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform 5; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform 6; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform 7; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, isoform 21; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, isoform 22; phosphatidylinositol-3; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; protein tyrosine phosphatase; protein tyrosine phosphatase and tensin homolog; PTEN; pten tumor suppressor; PTEN variant PTEN-K267Rfs*9; PTEN variant PTEN-L247*; PTEN variant PTEN-R234Afs*11; PTENepsilon; TEP1; unnamed protein product

          View more View less

          Gene Aliases: 10q23del; 2310035O07Rik; A130070J02Rik; AI463227; B430203M17Rik; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN; PTEN1; PTENbeta; PTENgama; TEP1

          View more View less

          UniProt ID: (Human) P60484, (Mouse) O08586

          View more View less

          Entrez Gene ID: (Human) 5728, (Mouse) 19211

          View more View less

          Function(s)
          phosphatidylinositol-3-phosphatase activity phosphoprotein phosphatase activity protein serine/threonine phosphatase activity protein tyrosine phosphatase activity protein binding beta-catenin binding lipid binding anaphase-promoting complex binding phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity hydrolase activity phosphatase activity enzyme binding PDZ domain binding inositol-1,3,4,5,6-pentakisphosphate 3-phosphatase activity identical protein binding inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity phosphatidylinositol phosphate phosphatase activity molecular function inhibitor activity ubiquitin-specific protease binding ubiquitin ligase activator activity magnesium ion binding platelet-derived growth factor receptor binding protein tyrosine/serine/threonine phosphatase activity protein kinase binding protein phosphatase
          Process(es)
          osteoblast differentiation protein dephosphorylation lipid metabolic process phosphatidylinositol biosynthetic process apoptotic process spindle assembly involved in female meiosis neuron-neuron synaptic transmission nervous system development synapse assembly central nervous system development heart development learning or memory locomotory behavior positive regulation of cell proliferation negative regulation of cell proliferation regulation of signal transduction positive regulation of macromolecule metabolic process negative regulation of cell communication negative regulation of epithelial to mesenchymal transition regulation of neuron projection development negative regulation of neuron projection development cell migration dentate gyrus development central nervous system neuron axonogenesis negative regulation of signaling negative regulation of cell migration adult behavior regulation of protein stability central nervous system myelin maintenance negative regulation of peptidyl-serine phosphorylation multicellular organismal response to stress social behavior maternal behavior protein kinase B signaling locomotor rhythm regulation of cell differentiation negative regulation of osteoblast differentiation negative regulation of cell size positive regulation of transcription from RNA polymerase II promoter negative regulation of organ growth phosphatidylinositol dephosphorylation forebrain morphogenesis cell motility negative regulation of axonogenesis protein stabilization negative regulation of developmental process negative regulation of cellular component organization negative regulation of multicellular organismal process negative regulation of keratinocyte migration regulation of cell cycle negative regulation of focal adhesion assembly regulation of protein kinase B signaling negative regulation of protein kinase B signaling rhythmic synaptic transmission synapse maturation prepulse inhibition dendritic spine morphogenesis regulation of biological quality cellular response to electrical stimulus negative regulation of excitatory postsynaptic potential presynaptic membrane assembly postsynaptic density assembly positive regulation of intracellular signal transduction negative regulation of cell cycle G1/S phase transition negative regulation of wound healing, spreading of epidermal cells negative regulation of vascular smooth muscle cell proliferation positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of G1/S transition of mitotic cell cycle positive regulation of excitatory postsynaptic potential negative regulation of cellular senescence negative regulation of synaptic vesicle clustering angiogenesis negative regulation of protein phosphorylation regulation of B cell apoptotic process memory dephosphorylation neuron projection development negative regulation of myelination negative regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle regulation of synaptic transmission, GABAergic regulation of cellular component size regulation of myeloid cell apoptotic process peptidyl-tyrosine dephosphorylation cellular response to decreased oxygen levels positive regulation of apoptotic process negative regulation of apoptotic process endothelial cell migration inositol phosphate dephosphorylation platelet-derived growth factor receptor signaling pathway regulation of axon regeneration cardiac muscle tissue development brain morphogenesis negative regulation of epithelial cell proliferation negative regulation of phagocytosis positive regulation of sequence-specific DNA binding transcription factor activity canonical Wnt signaling pathway male mating behavior long-term synaptic potentiation long term synaptic depression prostate gland growth negative regulation of dendritic spine morphogenesis positive regulation of ERK1 and ERK2 cascade cellular response to hypoxia negative regulation of ribosome biogenesis negative regulation of cell aging positive regulation of ubiquitin protein ligase activity positive regulation of apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.

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