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Invitrogen
GP6 Polyclonal Antibody
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FIGURE: 1 / 6
GP6 Antibody (PA5-20582) in ICC/IF
Immunofluorescence analysis of GP6 was performed using 70% confluent log phase K-562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GP6 Rabbit Polyclonal Antibody (Product # PA5-20582) at 10 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-20582
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 15 amino acid peptide near the carboxy terminus of the human GPVI.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is A-20 cell lysate.
PA5-20582 can be used with blocking peptide PEP-0702.
Target Information
Glycoprotein VI (GP6) is a 58-kD platelet membrane glycoprotein that plays a crucial role in the collagen-induced activation and aggregation of platelets. Upon injury to the vessel wall and subsequent damage to the endothelial lining, exposure of the subendothelial matrix to blood flow results in deposition of platelets. Collagen fibers are the most thrombogenic macromolecular components of the extracellular matrix, with collagen types I, III, and VI being the major forms found in blood vessels. Platelet interaction with collagen occurs as a 2-step procedure: (1) the initial adhesion to collagen is followed by (2) an activation step leading to platelet secretion, recruitment of additional platelets, and aggregation. In physiologic conditions, the resulting platelet plug is the initial hemostatic event limiting blood loss. However, exposure of collagen after rupture of atherosclerotic plaques is a major stimulus of thrombus formation associated with myocardial infarction or stroke.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: glycoprotein 5; glycoprotein 6; glycoprotein 6 (platelet); glycoprotein VI; platelet collagen receptor
Gene Aliases: 9830166G18Rik; BDPLT11; Gm469; GPIV; GPVI
UniProt ID: (Human) Q9HCN6, (Mouse) P0C191
Entrez Gene ID: (Human) 51206, (Rat) 684555, (Mouse) 243816
vasculature development
immune response-inhibiting cell surface receptor signaling pathway
peptidyl-tyrosine phosphorylation
collagen-activated tyrosine kinase receptor signaling pathway
collagen-activated signaling pathway
platelet aggregation
enzyme linked receptor protein signaling pathway
platelet activation
positive regulation of platelet aggregation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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