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Invitrogen
SPRED1 Polyclonal Antibody
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Product Details
PA5-20618
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 14 amino acid peptide near the center of the human Spred1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is human brain tissue lysate.
PA5-20618 can be used with blocking peptide PEP-0738.
Target Information
The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes; it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EVH1/Sprouty domain containing protein; protein phosphatase 1, regulatory subunit 147; Spred-1; sprouty protein with EVH-1 domain 1, related sequence; sprouty-related protein 1 with EVH-1 domain; Sprouty-related, EVH1 domain-containing protein 1; suppressor of Ras/MAPK activation; unnamed protein product
Gene Aliases: 5730461F13Rik; AL024345; AW047647; hSpred1; LGSS; NFLS; PPP1R147; spred-1; SPRED1
UniProt ID: (Human) Q7Z699, (Mouse) Q924S8
Entrez Gene ID: (Human) 161742, (Rat) 296072, (Mouse) 114715
regulation of signal transduction
negative regulation of epithelial to mesenchymal transition
negative regulation of angiogenesis
negative regulation of transforming growth factor beta receptor signaling pathway
regulation of MAPK cascade
negative regulation of MAPK cascade
positive regulation of DNA damage response, signal transduction by p53 class mediator
vasculogenesis involved in coronary vascular morphogenesis
negative regulation of ERK1 and ERK2 cascade
negative regulation of cell migration involved in sprouting angiogenesis
negative regulation of intracellular signal transduction
negative regulation of lens fiber cell differentiation
inactivation of MAPK activity
multicellular organism development
negative regulation of peptidyl-threonine phosphorylation
negative regulation of phosphatase activity
negative regulation of protein serine/threonine kinase activity
regulation of protein deacetylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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