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Invitrogen
ZNF281 Polyclonal Antibody
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FIGURE: 1 / 3
ZNF281 Antibody (PA5-21121) in ICC/IF
Immunofluorescent analysis of ZFP281 (red) in human embryonic stem cell H9 line grown on irradiated MEF-feeder layer. The cells were fixed with 4% paraformaldehyde at room temperature for 10 min and permeabilized with 0.25% Triton-X 100 for 5 min and blocked with the 10% BSA in PBS for 30 min at 37°C. Cells were stained with a ZFP281 polyclonal antibody (Product # PA5-21121) at a dilution of 1:200 in 3% BSA/PBS blocking buffer overnight at 4°C, and then incubated with a RRX-conjugated donkey anti-rabbit IgG secondary antibody at a dilution... View More
Product Details
PA5-21121
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 19 amino acid peptide near the carboxy terminus of human ZFP281.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is A-20 cell lysate.
PA5-21121 can be used with blocking peptide PEP-1235.
Target Information
ZFP281, also known as ZBP-99, is a member of a conserved family of transcription factors that interact with GC-rich promoters. It contains four Kruppel-like zinc fingers and is highly homologous to ZBP-89, another regulator of ornithine decarboxylase gene expression. ZFP281 also interacts with many other other transcription factors, such as Sp1 to repress vimentin gene transcription. ZFP281 has also been shown to control embryonic stem cell pluripotency by direct regulating specific target genes. ZFP281 phyically interacts with the transcription factos POU5F1, Sox2, and NANOG. Gene expression microarray anlysis indicated that some ZFP281 target genes were activated, while others were repressed upon knockdown of ZFP281 expression, showing that ZFP281 plays bifuctinal roles in regulating gene expression in stem cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GC-box-binding zinc finger protein 1; Transcription factor ZBP-99; unnamed protein product; Zinc finger DNA-binding protein 99; Zinc finger protein 281; ZNP-99 transcription factor
Gene Aliases: GZP1; ZBP-99; ZBP99; Zfp281; ZNF281; ZNP-99
UniProt ID: (Human) Q9Y2X9, (Mouse) Q99LI5
Entrez Gene ID: (Human) 23528, (Mouse) 226442
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
sequence-specific DNA binding
metal ion binding
sequence-specific double-stranded DNA binding
core promoter binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
nucleic acid binding
transcription corepressor activity
transcription regulatory region DNA binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
embryonic body morphogenesis
negative regulation of gene expression
cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
stem cell differentiation
transcription, DNA-templated
transcription from RNA polymerase II promoter
negative regulation of nucleic acid-templated transcription
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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