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Invitrogen
JMJD2C Polyclonal Antibody
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FIGURE: 1 / 13
JMJD2C Antibody (PA5-23065) in ICC/IF
Immunocytochemistry analysis of JMJD2C in NIH3T3 cells. Samples were incubated with JMJD2C polyclonal antibody (Product # PA5-23065) using a dilution of 2 µg/mL overnight at 4°C followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:1,000 dilution for 60 minutes. Cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-23065
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A partial recombinant protein made to an internal region of the human JMJD2C protein (within residues 450-600).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Target Information
Jumonji domain-containing protein 2C (JMJD2C)/ Lysine-specific demethylase 4C (KDM4C) is a histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3; thereby, playing a central role in histone code. KDM4C does not demethylate histone H3 'Lys-4', H3 'Lys-27', or H4 'Lys-20'. It demethylates trimethylated H3 "Lys-9' and H3 "Lys-36' residues. There is no activity on mono- or dimethylated residues. Demethylation of Lys residues generates formaldehyde and succinate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: [histone H3]-trimethyl-L-lysine(9) demethylase 4C; bA146B14.1; GASC-1; gene amplified in squamous cell carcinoma 1; gene amplified in squamous cell carcinoma 1 protein; JmjC domain-containing histone demethylation protein 3C; jumonji domain containing 2C; jumonji domain-containing protein 2C; lysine (K)-specific demethylase 4C; RP11-169L18.1; tudor domain containing 14C; unnamed protein product
Gene Aliases: 2410141F18Rik; GASC1; JHDM3C; JMJD2C; TDRD14C
UniProt ID: (Human) Q9H3R0, (Mouse) Q8VCD7
Entrez Gene ID: (Human) 23081, (Mouse) 76804
chromatin remodeling
positive regulation of cell proliferation
regulation of gene expression
androgen receptor signaling pathway
positive regulation of transcription from RNA polymerase II promoter
regulation of androgen receptor signaling pathway
blastocyst formation
positive regulation of gene expression
stem cell population maintenance
positive regulation of neuron differentiation
regulation of stem cell differentiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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