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Invitrogen
Nkx6.1 Polyclonal Antibody
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FIGURE: 1 / 4
Nkx6.1 Antibody (PA5-23070) in ICC/IF
Immunocytochemistry analysis of Nkx6.1 in INS-1 cells. Samples were incubated with Nkx6.1 polyclonal antibody (Product # PA5-23070) using a dilution of 1:250 followed by DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.
Product Details
PA5-23070
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A genomic peptide made to an internal region of the human Nkx6.1 protein (within residues 50-200).
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 30% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Target Information
NKX6.1 is a bi-functional transcription factor that has effects in both the central nervous system and in the pancreas. It has both expression and repression effects on genes, leading to the proper formation of motoneurons, oligodendrocytes, and insulin-secreting beta cells. It is known to interact with Pax6 and Pdx-1, probably at or near the 60 amino acid homeobox DNA-binding domain. Due to its obvious relationship to beta cells and CNS, NKX6.1 is becoming a more interesting target for therapies in diabetes and certain brain diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Drosophila NK transcription factor related, gene family 6, locus 1; Homeobox protein NK-6 homolog A; Homeobox protein Nkx-6.1; homeodomain transcription factor Nkx6.1; NK homeo box, family 6, member A; NK homeobox, family 6, A; NK6 transcription factor homolog A; NK6 transcription factor related locus 1; NK6 transcription factor related, locus 1
Gene Aliases: NKX6-1; NKX6.1; Nkx61; NKX6A
UniProt ID: (Human) P78426, (Mouse) Q99MA9, (Rat) O35762
Entrez Gene ID: (Human) 4825, (Mouse) 18096, (Rat) 65193
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
homeodomain transcription factor
negative regulation of transcription from RNA polymerase II promoter
type B pancreatic cell differentiation
pancreatic A cell differentiation
type B pancreatic cell development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
smoothened signaling pathway
response to xenobiotic stimulus
organ morphogenesis
glucose mediated signaling pathway
central nervous system neuron differentiation
neurogenesis
cell differentiation
neuron differentiation
regulation of axon extension
pancreas development
endocrine pancreas development
positive regulation of insulin secretion
response to nicotine
type B pancreatic cell proliferation
positive regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
oligodendrocyte differentiation
positive regulation of oligodendrocyte differentiation
negative regulation of oligodendrocyte differentiation
cellular response to cytokine stimulus
cellular response to peptide hormone stimulus
type B pancreatic cell maturation
positive regulation of type B pancreatic cell development
regulation of neuron migration
multicellular organism development
cell proliferation
regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification
regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification
negative regulation of glial cell differentiation
positive regulation of glial cell differentiation
response to drug
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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