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Invitrogen
H3K4me3 Polyclonal Antibody
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FIGURE: 1 / 6
H3K4me3 Antibody (PA5-27029) in WB
Western blot analysis of H3K4me3 in HeLa histone prep (A), NIH 3T3 histone prep (B), HeLa whole cell extract (C), and NIH 3T3 whole cell extract (D). Samples were incubated in H3K4me3 polyclonal antibody (Product # PA5-27029 using a dilution of 1 µg/mL. Observed molecular weight is ~15 kDa.
Product Details
PA5-27029
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic trimethylated peptide surrounding Lysine 4 of human Histone H3.2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with many species including rat, chicken, xenopus, drosophila, and plant based on 100% sequence homology.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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